| Literature DB >> 8137953 |
Abstract
Expression of the cDNA for Aequorea green fluorescent protein in E. coli yielded a fused protein with fluorescence excitation and emission spectra virtually identical to those of the native green fluorescent protein. Further, a solution of the protein, when mixed with aequorin and calcium ion, emitted a greenish luminescence characteristic of the in vivo luminescence of the animal, indicating a radiationless energy transfer to the protein.Entities:
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Year: 1994 PMID： 8137953 DOI： 10.1016/0014-5793(94)80472-9
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124