| Literature DB >> 8137913 |
V I Ugarov1, G Y Jung, A B Chetverin, A S Spirin.
Abstract
Expression of dihydrofolate reductase (DHFR) and chloramphenicol acetyltransferase (CAT) mRNAs in cell-free Escherichia coli translation systems is greatly enhanced as a result of their insertion into RQ135 RNA, a naturally occurring satellite of phage Q beta. The enhancement is due to protection of the recombinant mRNAs against endogenous ribonucleases and to an increased initial rate of translation in the case of the RQ-CAT mRNA.Entities:
Mesh：
Substances：
Year: 1994 PMID： 8137913 DOI： 10.1016/0014-5793(94)80255-6
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124