Literature DB >> 8136027

Purification and partial sequence of proteins involved in the cholic acid transport into rat liver hepatocytes.

A Hoffmann1, S Müllner, K Ziegler, H Fasold.   

Abstract

Two proteins, in previous work labeled by affinity markers derived from taurocholic acid, were purified and partially sequenced. Antibodies were raised against purified proteins, and cross-reactions were carefully checked. The influence of these antibodies upon taurocholic acid import into vesicles from rat liver plasma membranes was measured, and showed a distinct inhibition of transport in the case of the 54 kD protein.

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Year:  1993        PMID: 8136027     DOI: 10.1007/bf01024935

Source DB:  PubMed          Journal:  J Protein Chem        ISSN: 0277-8033


  13 in total

1.  High resolution two-dimensional electrophoresis of proteins.

Authors:  P H O'Farrell
Journal:  J Biol Chem       Date:  1975-05-25       Impact factor: 5.157

2.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

3.  A micromethod for complete removal of dodecyl sulfate from proteins by ion-pair extraction.

Authors:  L E Henderson; S Oroszlan; W Konigsberg
Journal:  Anal Biochem       Date:  1979-02       Impact factor: 3.365

4.  Two-dimensional gel electrophoresis of membrane proteins.

Authors:  G F Ames; K Nikaido
Journal:  Biochemistry       Date:  1976-02-10       Impact factor: 3.162

5.  Isolation of microgram quantities of proteins from polyacrylamide gels for amino acid sequence analysis.

Authors:  M W Hunkapiller; E Lujan; F Ostrander; L E Hood
Journal:  Methods Enzymol       Date:  1983       Impact factor: 1.600

6.  Immunization, isolation of immunoglobulins, estimation of antibody titre.

Authors:  N Harboe; A Ingild
Journal:  Scand J Immunol Suppl       Date:  1973

7.  3'-Isothiocyanatobenzamido[3H]cholate, a new affinity label for hepatocellular membrane proteins responsible for the uptake of both bile acids and phalloidin.

Authors:  K Ziegler; M Frimmer; S Müllner; H Fasold
Journal:  Biochim Biophys Acta       Date:  1984-06-13

8.  Structural analysis and specific expression of microsomal cytochrome P-450(M-1) mRNA in male rat livers.

Authors:  H Yoshioka; K Morohashi; K Sogawa; T Miyata; K Kawajiri; T Hirose; S Inayama; Y Fujii-Kuriyama; T Omura
Journal:  J Biol Chem       Date:  1987-02-05       Impact factor: 5.157

9.  Bile acid binding proteins in hepatocellular membranes of newborn and adult rats. Identification of transport proteins with azidobenzamidotauro[14C]cholate ([14C]ABATC).

Authors:  K Ziegler; M Frimmer; S Müllner; H Fasold
Journal:  Biochim Biophys Acta       Date:  1989-04-14

10.  Characterization of the bile acid transport system in normal and transformed hepatocytes. Photoaffinity labeling of the taurocholate carrier protein.

Authors:  P von Dippe; D Levy
Journal:  J Biol Chem       Date:  1983-07-25       Impact factor: 5.157

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