Literature DB >> 8135826

Effect of myosin light chain kinase inhibitor wortmannin on the wound repair of cultured gastric mucosal cells.

S Watanabe1, X E Wang, M Hirose, N Sato.   

Abstract

The effects of myosin light chain kinase inhibitor wortmannin on wound restoration were investigated using a culture cell model. Rabbit gastric mucosal cells formed confluent monolayer cell sheets within 48h. Cell sheets were wounded to create wounds of uniform size. The restoration was monitored by a time-lapse video disc recorder and was analyzed. The wound was completely restored with early cell migration and late proliferation in controls. Wortmannin inhibited cell migration, thus inhibiting proliferation. Results indicate that the cytoskeleton plays a key role in the restoration of gastric mucosa.

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Year:  1994        PMID： 8135826     DOI： 10.1006/bbrc.1994.1300

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  6 in total

1.  Ammonia, hydrogen peroxide, and monochloramine retard gastric epithelial restoration in rabbit cultured cell model.

Authors:  K Sato; S Watanabe; T Yoshizawa; M Hirose; T Murai; N Sato
Journal:  Dig Dis Sci       Date:  1999-12       Impact factor: 3.199

2.  Effects of epidermal growth factor and insulin on migration and proliferation of primary cultured rabbit gastric epithelial cells.

Authors:  K Maehiro; S Watanabe; M Hirose; R Iwazaki; H Miwa; N Sato
Journal:  J Gastroenterol       Date:  1997-10       Impact factor: 7.527

3.  Migration of primary cultured rabbit gastric epithelial cells requires intact protein kinase C and Ca2+/calmodulin activity.

Authors:  Tuula Ranta-Knuuttila; Tuula Kiviluoto; Harri Mustonen; Pauli Puolakkainen; Sumio Watanabe; Nobuhiro Sato; Eero Kivilaakso
Journal:  Dig Dis Sci       Date:  2002-05       Impact factor: 3.199

4.  Effect of mechanical strain on gastric cellular migration and proliferation during mucosal healing: role of Rho dependent and Rac dependent cytoskeletal reorganisation.

Authors:  T Osada; S Watanabe; H Tanaka; M Hirose; A Miyazaki; N Sato
Journal:  Gut       Date:  1999-10       Impact factor: 23.059

5.  Expression of gene for EIIIA- and EIIIB- fibronectin, fetal types of fibronectin, during gastric ulcer healing in rats.

Authors:  K Tominaga; K Higuchi; T Watanabe; Y Fujiwara; S Kim; T Arakawa; H Iwao; T Kuroki
Journal:  Dig Dis Sci       Date:  2001-02       Impact factor: 3.199

6.  Cell injury triggers actin polymerization to initiate epithelial restitution.

Authors:  Eitaro Aihara; Neisha M Medina-Candelaria; Hikaru Hanyu; Andrea L Matthis; Kristen A Engevik; Christine B Gurniak; Walter Witke; Jerrold R Turner; Tongli Zhang; Marshall H Montrose
Journal:  J Cell Sci       Date:  2018-08-20       Impact factor: 5.285
  6 in total

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