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Literature DB >> 813335

A decalification method for ultrastructure of echinoderm tissues.

Abstract

Decalcification of echinoderm tissues for electron microscopy can be achieved after glutaraldehyde-osmium fixation by treatment with a 1:1 mixture of 2% ascorbic acid and 0.3 M NaCl for 12-24 hours. Electron photomicrographs of material decalcified by this procedure are superior to those from EDTA-treated tissue and show few of the deleteriour effects produced by EDTA.

Entities: Chemical

Mesh：

Substances：
Edetic Acid
Ascorbic Acid

Year: 1975 PMID： 813335 DOI： 10.3109/10520297509117086

Source DB: PubMed Journal: Stain Technol ISSN： 0038-9153

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Cited

12 in total

1. The effect of decalcification on the microstructure of articular cartilage assessed by 2H double quantum filtered spectroscopic MRI.

Authors: Keren Keinan-Adamsky; Hadassah Shinar; Gil Navon
Journal: MAGMA Date: 2005-11-22 Impact factor: 2.310

2. Neurobiology of the gorgonian coelenterates, Muricea californica and Lophogorgia chilensis.

Authors: R A Satterlie; J F Case
Journal: Cell Tissue Res Date: 1978-03-13 Impact factor: 5.249

3. Collagen and proteoglycan in a sea urchin ligament with mutable mechanical properties.

Authors: J A Trotter; T J Koob
Journal: Cell Tissue Res Date: 1989-12 Impact factor: 5.249

4. Mechanisms of arm-tip regeneration in the sea star, Leptasterias hexactis.

Authors: Philip V Mladenov; Brent Bisgrove; Satish Asotra; Robert D Burke
Journal: Rouxs Arch Dev Biol Date: 1989-05

5. Localization of S1- and S2-like immunoreactivity in the nervous system of the brittle star Amphipholis squamata (Delle Chiaje 1828).

Authors: N de Bremaeker; D Deheyn; M C Thorndyke; F Baguet; J Mallefet
Journal: Proc Biol Sci Date: 1997-05-22 Impact factor: 5.349

6. Localization and synthesis of type III collagen and fibronectin in human reparative dentine. Immunoperoxidase and immunogold staining.

Authors: H Magloire; A Joffre; D J Hartmann
Journal: Histochemistry Date: 1988

A decalification method for ultrastructure of echinoderm tissues.

1. The effect of decalcification on the microstructure of articular cartilage assessed by 2H double quantum filtered spectroscopic MRI.

2. Neurobiology of the gorgonian coelenterates, Muricea californica and Lophogorgia chilensis.

3. Collagen and proteoglycan in a sea urchin ligament with mutable mechanical properties.

4. Mechanisms of arm-tip regeneration in the sea star, Leptasterias hexactis.

5. Localization of S1- and S2-like immunoreactivity in the nervous system of the brittle star Amphipholis squamata (Delle Chiaje 1828).

6. Localization and synthesis of type III collagen and fibronectin in human reparative dentine. Immunoperoxidase and immunogold staining.

7. Light- and electron-microscopic study of the endolymphatic sac of the tree frog, Hyla arborea japonica.

8. Molecular structure and functional morphology of echinoderm collagen fibrils.

9. The giant neurone system in Ophiuroids. II. The hyponeural motor tracts.

10. The giant neurone system in ophiuroids. III. The detailed connections of the circumoral nerve ring.