| Literature DB >> 8133282 |
S J Hardy1, A Ferrante, B S Robinson, D W Johnson, A Poulos, K J Clark, A W Murray.
Abstract
A variety of fatty acids including the cis-polyunsaturated very-long-chain fatty acids (VLCFA) (> 22 carbon atoms) common in retina, spermatozoa, and brain were examined for their ability to activate protein kinase C (PKC) purified from rat brain. Arachidonic [20:4(n-6)], eicosapentaenoic [20:5(n-3)], and docosahexaenoic [22:6(n-3)] acids as well as the VLCFA dotriacontatetraenoic [32:4(n-6)] and tetratriacontahexaenoic [34:6(n-3)] were equally capable of activating PKC in vitro with maximal activity being between 25 and 50 microM. The phorbol ester 12-O-tetradecanoylphorbol 13-acetate further enhanced the in vitro activation of PKC when added to the protein kinase assay system with the fatty acids. The fully saturated arachidic acid (20:0) was inactive in both assay systems. The potential significance of the in vitro activation of PKC by the VLCFA is discussed.Entities:
Mesh：
Substances：
Year: 1994 PMID： 8133282 DOI： 10.1046/j.1471-4159.1994.62041546.x
Source DB: PubMed Journal: J Neurochem ISSN： 0022-3042 Impact factor: 5.372