OBJECTIVE: To study the effects of N-chlorotaurine on collagenase activity, as a model of the effects of neutrophil activation in inflammatory arthritis. METHODS: Collagen degradation by collagenase was measured by the release of acid-soluble counts from 3H-collagen. RESULTS: N-chlorotaurine inhibited the degradation of collagen by bacterial collagenase. This result is explained by a direct inhibition/inactivation of collagenase, since N-chlorotaurine had no effect on the proteolytic susceptibility of collagen itself. The effect appears to be specific to N-chlorotaurine since N-chloroalanine, N-chloroleucine, and HOCl/OCl- failed to inhibit collagenase; in fact, N-chloroalanine and N-chloroleucine actually increased the proteolytic susceptibility of collagen. CONCLUSION: N-chlorotaurine may minimize damage to cartilaginous joint structures in inflammatory arthritis by inhibiting/inactivating collagenase.
OBJECTIVE: To study the effects of N-chlorotaurine on collagenase activity, as a model of the effects of neutrophil activation in inflammatory arthritis. METHODS: Collagen degradation by collagenase was measured by the release of acid-soluble counts from 3H-collagen. RESULTS:N-chlorotaurine inhibited the degradation of collagen by bacterial collagenase. This result is explained by a direct inhibition/inactivation of collagenase, since N-chlorotaurine had no effect on the proteolytic susceptibility of collagen itself. The effect appears to be specific to N-chlorotaurine since N-chloroalanine, N-chloroleucine, and HOCl/OCl- failed to inhibit collagenase; in fact, N-chloroalanine and N-chloroleucine actually increased the proteolytic susceptibility of collagen. CONCLUSION:N-chlorotaurine may minimize damage to cartilaginous joint structures in inflammatory arthritis by inhibiting/inactivating collagenase.
Authors: K Lorenz; D Mayer; G Bruhn; B Noack; M Brecx; C Heumann; H Toutenburg; L Netuschil; M Nagl; W Gottardi; T Hoffmann Journal: Clin Oral Investig Date: 2008-06-27 Impact factor: 3.573
Authors: Yinian Zhang; Huateng Dong; Daniel P Seeburg; Gregory R Wojtkiewicz; Peter Waterman; Benjamin Pulli; Reza Forghani; Muhammad Ali; Yoshiko Iwamoto; Filip K Swirski; John W Chen Journal: Mol Neurobiol Date: 2018-05-28 Impact factor: 5.590