| Literature DB >> 8129475 |
D E Minnikin1, R C Bolton, S Hartmann, G S Besra, P A Jenkins, A I Mallet, E Wilkins, A M Lawson, M Ridell.
Abstract
An integrated method is described for the sensitive detection of tuberculostearic, mycocerosic and mycolic acids in infected materials from tuberculosis patients. Tuberculostearic acid is analysed by two-dimensional gas chromatography of pentafluorobenzyl esters, the key component being switched from a short non-polar column to a high resolution polar column with final electron capture detection. Mycocerosic acids are identified by simple one-dimensional electron capture gas chromatography of pentafluorobenzyl esters, with the use of negative-ion chemical ionisation gas chromatography in indecisive cases. Conversion of mycolic acids to anthrylmethyl esters produces compounds which are suitable for sensitive detection by fluorescence high-performance liquid chromatography. Application of all three of these methods to sputum samples from tuberculosis patients gave profiles characteristic of Mycobacterium tuberculosis.Entities:
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Year: 1993 PMID： 8129475
Source DB: PubMed Journal: Ann Soc Belg Med Trop ISSN： 0772-4128