Effects of hyaluronan on collagen fibrillar matrix contraction by fibroblasts.

Hyaluronan, found in high concentrations in fetal tissues, appears to have a major role in preventing scar formation in fetal wounds. Nevertheless, its role in inhibiting wound contractures associated with scar formation has not been clearly demonstrated. Our current study evaluated the effects of hyaluronan using an in vitro floating collagen fibrillar matrix (CFM) contraction model. The results demonstrated that the contraction of CFM by fibroblasts was significantly reduced when high concentrations (> 1 mg/mL) of hyaluronan were present in the media. This phenomenon is unique to hyaluronan, because chondroitin sulfate was ineffective in this connection. Fibroblast migration and proliferation studies indicated that high concentrations of hyaluronan stimulated cell migration and had no cytotoxic effects. Some possible mechanisms by which high concentrations of hyaluronan reduced CFM contraction by fibroblasts were proposed. Because the viscosity of a hyaluronan solution is much greater than that of chondroitin sulfate, and this increases with concentration, we investigated whether this property in itself was an important factor in inhibiting CFM contraction. No direct correlation was found between the viscosity of glycosaminoglycans and their ability to reduce CFM contraction.