Literature DB >> 8125050

The focusing positions of polypeptides in immobilized pH gradients can be predicted from their amino acid sequences.

B Bjellqvist1, G J Hughes, C Pasquali, N Paquet, F Ravier, J C Sanchez, S Frutiger, D Hochstrasser.   

Abstract

The focusing positions in narrow range immobilized pH gradients of 29 polypeptides of known amino acid sequence were determined under denaturing conditions. The isoelectric points of the proteins calculated from their amino acid sequences matched with good accuracy the experimentally determined pI values. We show the advantages of being able to predict the position of a protein of known structure within a two-dimensional gel.

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Year:  1993        PMID： 8125050     DOI： 10.1002/elps.11501401163

Source DB:  PubMed          Journal:  Electrophoresis        ISSN： 0173-0835            Impact factor:   3.535


  245 in total

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