Effects of the antibiotic resistomycin on the synthesis of macromolecules.

Resistomycin preferentially inhibits RNA synthesis in comparison to DNA and protein synthesis in intact bacterial cells. Studies with cell-free systems have shown that the antibiotic interferes with DNA and RNA synthesis, while protein synthesis is inhibited to a much lesser extent. Detailed studies in cell-free systems indicate an interaction of resistomycin with DNA- and RNA polymerase. In the case of RNA polymerase this was proved by CD measurements, whereas no interaction of the antibiotic with DNA, RNA, and homopolynucleotides could be found. One can conclude that the binding of the antibiotic to RNA polymerase is the basis for its interference with RNA synthesis.