| Literature DB >> 8119379 |
V Calenda1, P Graber, J F Delamarter, J C Chermann.
Abstract
In previous studies (1-4), we described disrupted hematopoiesis in long-term human bone marrow cultures infected with HIV. Conditioned medium (CM) from HIV-1 non-productively infected liquid cultures inhibits the proliferation of granulo-monocytic progenitor cells (CFU-GM) in clonogenic assays. A growth-inhibitory soluble factor was therefore suspected and we report here that this factor has been identified as the nef gene product. These nef-containing supernatants are specifically neutralized by anti-nef antibodies and recombinant nef has the same growth inhibitory properties. We used a nef-deficient virus to confirm that nef protein is responsible for the in vitro growth inhibition of CFU-GM.Entities:
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Year: 1994 PMID： 8119379 DOI： 10.1111/j.1600-0609.1994.tb01294.x
Source DB: PubMed Journal: Eur J Haematol ISSN： 0902-4441 Impact factor: 2.997