| Literature DB >> 8118403 |
S Pecharatana1, M A Pickett, P J Watt, M E Ward.
Abstract
A single-tube nested PCR able to discriminate between ocular infection with Chlamydia trachomatis of serovar A, B, or C is described. The method uses genotype-specific primers labeled with different fluorochromes, "drop-in/drop-out" PCR amplification, and product analysis on an Applied Biosystems 373A DNA Sequencer using GENESCAN 672 software. The system readily detected mixed infection with serotypes A and B within the same eye. This strategy provides a rapid genotyping method applicable to a wide variety of epidemiological studies.Entities:
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Year: 1993 PMID： 8118403 DOI： 10.1101/gr.3.3.200
Source DB: PubMed Journal: PCR Methods Appl ISSN： 1054-9803