Literature DB >> 8117799

Application of a DNA-DNA hybridization method for detection of Campylobacter jejuni in chicken feces.

T Chuma1, T Yamada, K Okamoto, H Yugi, T Ohya.   

Abstract

A direct colony hybridization method was used for the detection of Campylobacter jejuni in chicken feces. The biotin-labeled DNA prepared from the whole genome DNA of C. jejuni subsp. jejuni ATCC 33560 reacted well with homologous DNA and slightly with C. coli DNA. The method with the probe was found to be sensitive enough to detect a small number (10(2) CFU/g) of C. jejuni in chicken feces which contained a large number of background flora. It was suggested that this simple and sensitive method was useful for a wide survey of C. jejuni.

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Year:  1993        PMID: 8117799     DOI: 10.1292/jvms.55.1027

Source DB:  PubMed          Journal:  J Vet Med Sci        ISSN: 0916-7250            Impact factor:   1.267


  2 in total

1.  Genotypic Diversity among Campylobacter jejuni Isolates in a Commercial Broiler Flock.

Authors:  L M Thomas; K A Long; R T Good; M Panaccio; P R Widders
Journal:  Appl Environ Microbiol       Date:  1997-05       Impact factor: 4.792

2.  Effective recovery of bacterial DNA and percent-guanine-plus-cytosine-based analysis of community structure in the gastrointestinal tract of broiler chickens.

Authors:  J H Apajalahti; L K Särkilahti; B R Mäki; J P Heikkinen; P H Nurminen; W E Holben
Journal:  Appl Environ Microbiol       Date:  1998-10       Impact factor: 4.792

  2 in total

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