Literature DB >> 8117434

Contamination of genetically engineered CHO-cells by epizootic haemorrhagic disease virus (EHDV).

H Rabenau1, V Ohlinger, J Anderson, B Selb, J Cinatl, W Wolf, J Frost, P Mellor, H W Doerr.   

Abstract

The characterization of a contaminating virus which was detected in genetically-engineered Chinese hamster ovary (CHO) cells during the production of biologicals is described in the present paper. Under electron microscopy, the contaminating virus had a morphology resembling that of an orbivirus. The relationship was confirmed by nucleic acid analysis which showed a RNA segment pattern characteristic of orbiviruses. With an immunoperoxidase staining of monolayer cells and through sero-neutralization tests the virus was identified as being identical to Epizootic Haemorrhagic Disease Virus (EHDV), isolate 318 (untyped) from Bahrain. Potential sources of the contaminating virus and feasible procedures to avoid adventitous virus infections in cell cultures are discussed.

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Year:  1993        PMID： 8117434     DOI： 10.1006/biol.1993.1077

Source DB:  PubMed          Journal:  Biologicals        ISSN： 1045-1056            Impact factor:   1.856


  8 in total

1.  RT-PCR assays for seven serotypes of epizootic haemorrhagic disease virus & their use to type strains from the Mediterranean region and North America.

Authors:  Narender S Maan; Sushila Maan; Kyriaki Nomikou; Donna J Johnson; Mehdi El Harrak; Hafsa Madani; Hagai Yadin; Serife Incoglu; Kadir Yesilbag; Andrew B Allison; David E Stallknecht; Carrie Batten; Simon J Anthony; Peter P C Mertens
Journal:  PLoS One       Date:  2010-09-17       Impact factor: 3.240

2.  Serum-free produced Bovine Herpesvirus type 1 and Bovine Parainfluenza type 3 virus vaccines are efficacious and safe.

Authors:  B Makoschey; J R Patel; P T J A van Gelder
Journal:  Cytotechnology       Date:  2002-09       Impact factor: 2.058

3.  Vesivirus 2117 capsids more closely resemble sapovirus and lagovirus particles than other known vesivirus structures.

Authors:  Michaela Conley; Edward Emmott; Richard Orton; David Taylor; Daniel G Carneiro; Kazuyoshi Murata; Ian G Goodfellow; Grant S Hansman; David Bhella
Journal:  J Gen Virol       Date:  2017-02-06       Impact factor: 3.891

4.  Combating viral contaminants in CHO cells by engineering innate immunity.

Authors:  Austin W T Chiang; Shangzhong Li; Benjamin P Kellman; Gouri Chattopadhyay; Yaqin Zhang; Chih-Chung Kuo; Jahir M Gutierrez; Faezeh Ghazi; Hana Schmeisser; Patrice Ménard; Sara Petersen Bjørn; Bjørn G Voldborg; Amy S Rosenberg; Montserrat Puig; Nathan E Lewis
Journal:  Sci Rep       Date:  2019-06-20       Impact factor: 4.379

5.  Virus susceptibility of Chinese hamster ovary (CHO) cells and detection of viral contaminations by adventitious agent testing.

Authors:  Andreas Berting; Maria R Farcet; Thomas R Kreil
Journal:  Biotechnol Bioeng       Date:  2010-07-01       Impact factor: 4.530

Review 6.  Validation of biopharmaceutical purification processes for virus clearance evaluation.

Authors:  Allan Darling
Journal:  Mol Biotechnol       Date:  2002-05       Impact factor: 2.695

7.  Development of real-time RT-qPCR assays for the typing of two novel bluetongue virus genotypes derived from sheeppox vaccine.

Authors:  Simon King; John Flannery; Carrie Batten; Paulina Rajko-Nenow
Journal:  J Virol Methods       Date:  2021-09-15       Impact factor: 2.014

8.  Virus contaminations of cell cultures - A biotechnological view.

Authors:  O-W Merten
Journal:  Cytotechnology       Date:  2002-07       Impact factor: 2.058
  8 in total

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