Literature DB >> 811631

A simplified method of specimen preparation for X-ray microanalysis of muscle and blood cells.

R Yarom, C Maunder, M Scripps, T A Hall, V Dubowitz.   

Abstract

The effect of various steps in the preparation of muscle and blood cells for electron microscopic X-ray microanalysis, was studied with reference to calcium, zinc and copper levels. It was found that these elements are readily detectable in material fixed in glutaraldehyde and embedded directly in Epon 812. Alcohol dehydration, buffer rinses and post-osmication caused marked depletion of all elements examined.

Entities:  

Mesh：

Substances：

Year:  1975        PMID： 811631     DOI： 10.1007/BF00508050

Source DB:  PubMed          Journal:  Histochemistry        ISSN： 0301-5564


  12 in total

1.  Calcium in myonuclei: electron microprobe X-ray analysis.

Authors:  R Yarom; T A Hall; P D Peters
Journal:  Experientia       Date:  1975-02-15

2.  Electron probe analysis of the calcium distribution in cells of the embryonic chick chorioallantoic membrane. I. A critical evaluation of techniques.

Authors:  J R Coleman; A R Terepka
Journal:  J Histochem Cytochem       Date:  1972-06       Impact factor: 2.479

3.  Fixation in aldehydes. A study on the influence of the fixative, buffer, and osmolarity upon the fixation of the rat retina.

Authors:  K E Rasmussen
Journal:  J Ultrastruct Res       Date:  1974-01

4.  Effect of specimen preparation on intracellular myocardial calcium. Electron microscopic x-ray microanalysis.

Authors:  R Yarom; P D Peters; M Scripps; S Rogel
Journal:  Histochemie       Date:  1974-02-06

5.  Electron cytochemistry of calcium uptake in the fragmented sarcoplasmic reticulum.

Authors:  B Agostini; W Hasselbach
Journal:  Histochemie       Date:  1971

6.  Copper and zinc concentration in the plasma of leukaemic children.

Authors:  H T Delves; F W Alexander; H Lay
Journal:  Br J Haematol       Date:  1973-04       Impact factor: 6.998

7.  Effect of glutaraldehyde and urea embedding on intracellular ionic elements. X-ray microanalysis of skeletal muscle and myocardium.

Authors:  R Yarom; P D Peters; T A Hall
Journal:  J Ultrastruct Res       Date:  1974-12

8.  Influence of glutaraldehyde and-or osmium tetroxide on cell volume, ion content, mechanical stability, and membrane permeability of Ehrlich ascites tumor cells.

Authors:  A Penttila; H Kalimo; B F Trump
Journal:  J Cell Biol       Date:  1974-10       Impact factor: 10.539

9.  Calcium binding to intestinal membranes.

Authors:  J L Oschman; B J Wall
Journal:  J Cell Biol       Date:  1972-10       Impact factor: 10.539

10.  The intracellular site of calcium activaton of contraction in frog skeletal muscle.

Authors:  S Winegrad
Journal:  J Gen Physiol       Date:  1970-01       Impact factor: 4.086
View more
  5 in total

1.  Studies on the mechanisms of neurulation in the chick: the intracellular distribution of Ca++.

Authors:  R G Nagele; J F Pietrolungo; H Lee
Journal:  Experientia       Date:  1981-03-15

2.  Microfluorometric investigations of chromatin structure. II. Mordant fluorochroming with ions that complex with morin.

Authors:  R R Cowden; S K Curtis
Journal:  Histochemistry       Date:  1981

3.  X-ray microanalysis of isolated perfused rat hearts.

Authors:  D Tzivoni; R Yarom; M Fields; T A Hall; M S Gotsman
Journal:  Basic Res Cardiol       Date:  1978 Mar-Apr       Impact factor: 17.165

4.  The elemental composition of the chloragosomes of two earthworm species (Lumbricus terrestris and Allolobophora longa) determined by electron probe X-ray microanalysis of freeze-dried cryosections.

Authors:  A J Morgan; C Winters
Journal:  Histochemistry       Date:  1982

5.  Localised concentrations of elements in hamster cardiomyopathy. Electron microscopic x-ray microanalysis of normal and sick myocardia.

Authors:  R Yarom; T A Hall; C M Oakley
Journal:  Basic Res Cardiol       Date:  1977 Nov-Dec       Impact factor: 17.165
  5 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.