Literature DB >> 8113200

Construction of a modified penicillin-binding protein 2a from methicillin-resistant Staphylococcus aureus and purification by immobilized metal affinity chromatography.

C Y Wu1, L C Blaszczak, M C Smith, P L Skatrud.   

Abstract

The mecA-27r gene, which encodes PBP2a-27r, was modified by site-specific mutagenesis, resulting in replacement of the N-terminal membrane anchor with a short chelating peptide (CP-PBP2a-27r). CP-PBP2a-27r retained the same binding affinity for beta-lactam antibiotics as the wild-type enzyme. Approximately 95% pure CP-PBP2a-27r was recovered in a single step by use of chelating-peptide-immobilized metal ion affinity chromatography.

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Year:  1994        PMID： 8113200      PMCID： PMC205227          DOI： 10.1128/jb.176.5.1539-1541.1994

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  11 in total

Review 1.  Consequences of the interaction of beta-lactam antibiotics with penicillin binding proteins from sensitive and resistant Staphylococcus aureus strains.

Authors:  H Labischinski
Journal:  Med Microbiol Immunol       Date:  1992       Impact factor: 3.402

2.  World-wide antibiotic resistance in methicillin-resistant Staphylococcus aureus.

Authors:  P A Maple; J M Hamilton-Miller; W Brumfitt
Journal:  Lancet       Date:  1989-03-11       Impact factor: 79.321

3.  Nosocomial infection surveillance, 1984.

Authors:  T C Horan; J W White; W R Jarvis; T G Emori; D H Culver; V P Munn; C Thornsberry; D R Olson; J M Hughes
Journal:  MMWR CDC Surveill Summ       Date:  1986

4.  Site-directed mutagenesis of the mecA gene from a methicillin-resistant strain of Staphylococcus aureus.

Authors:  C Y Wu; W E Alborn; J E Flokowitsch; J Hoskins; S Unal; L C Blaszczak; D A Preston; P L Skatrud
Journal:  J Bacteriol       Date:  1994-01       Impact factor: 3.490

5.  Rapid and efficient site-specific mutagenesis without phenotypic selection.

Authors:  T A Kunkel
Journal:  Proc Natl Acad Sci U S A       Date:  1985-01       Impact factor: 11.205

6.  Low-affinity penicillin-binding protein associated with beta-lactam resistance in Staphylococcus aureus.

Authors:  B J Hartman; A Tomasz
Journal:  J Bacteriol       Date:  1984-05       Impact factor: 3.490

7.  Construction of a water-soluble form of penicillin-binding protein 2a from a methicillin-resistant Staphylococcus aureus isolate.

Authors:  C Y Wu; J Hoskins; L C Blaszczak; D A Preston; P L Skatrud
Journal:  Antimicrob Agents Chemother       Date:  1992-03       Impact factor: 5.191

8.  Presence of an additional penicillin-binding protein in methicillin-resistant Staphylococcus epidermidis, Staphylococcus haemolyticus, Staphylococcus hominis, and Staphylococcus simulans with a low affinity for methicillin, cephalothin, and cefamandole.

Authors:  J Pierre; R Williamson; M Bornet; L Gutmann
Journal:  Antimicrob Agents Chemother       Date:  1990-09       Impact factor: 5.191

9.  Molecular cloning of the gene of a penicillin-binding protein supposed to cause high resistance to beta-lactam antibiotics in Staphylococcus aureus.

Authors:  M Matsuhashi; M D Song; F Ishino; M Wachi; M Doi; M Inoue; K Ubukata; N Yamashita; M Konno
Journal:  J Bacteriol       Date:  1986-09       Impact factor: 3.490

10.  One-step purification of recombinant human papillomavirus type 16 E7 oncoprotein and its binding to the retinoblastoma gene product.

Authors:  M S Kasher; M Wakulchik; J A Cook; M C Smith
Journal:  Biotechniques       Date:  1993-04       Impact factor: 1.993
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