Literature DB >> 8113166

Cytosylglucuronic acid synthase (cytosine: UDP-glucuronosyltransferase) from Streptomyces griseochromogenes, the first prokaryotic UDP-glucuronosyltransferase.

S J Gould1, J Guo.   

Abstract

Cytosylglucuronic acid synthase (cytosine: UDP-glucuronosyltransferase), the first prokaryotic UDP-GT and a key enzyme in the biosynthesis of the antibiotic blasticidin S, was purified 870-fold. It has optimum activity at a pH of 8.4 to 8.6, Kms of 6.0 (UDP-glucuronic acid) and 243 (cytosine) microM, and a maximum rate of metabolism of 14.6 mumol/min/mg. The apparent M(r) is 43,000. Activity was slightly enhanced by Mg2+ or Ca2+ but was not inhibited by EDTA. Activity was strongly inhibited by UDP. Cytosylglucuronic acid differs from eukaryotic UDP-glucuronosyltransferases in being a soluble protein with no apparent phospholipid requirement.

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Year:  1994        PMID： 8113166      PMCID： PMC205190          DOI： 10.1128/jb.176.5.1282-1286.1994

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  25 in total

1.  THE IMPROVED ASSAY METHOD OF BLASTICIDIN S.

Authors:  T SHOMURA; M INOUE; T NIIDA; T HARA
Journal:  J Antibiot (Tokyo)       Date:  1964-11       Impact factor: 2.649

2.  Blasticidin S, a new antibiotic.

Authors:  S TAKEUCHI; K HIRAYAMA; K UEDA; H SAKAI; H YONEHARA
Journal:  J Antibiot (Tokyo)       Date:  1958-01       Impact factor: 2.649

3.  Specific inhibition of TMV-RNA synthesis by blasticidin S.

Authors:  A Hirai; S G Wildman; T Hirai
Journal:  Virology       Date:  1968-12       Impact factor: 3.616

4.  A rapid, sensitive, and versatile assay for protein using Coomassie brilliant blue G250.

Authors:  J J Sedmak; S E Grossberg
Journal:  Anal Biochem       Date:  1977-05-01       Impact factor: 3.365

5.  Factors modulating the catalytic specificity of a pure form of UDP-glucuronyltransferase.

Authors:  J Magdalou; Y Hochman; D Zakim
Journal:  J Biol Chem       Date:  1982-11-25       Impact factor: 5.157

6.  Metabolism of xenobiotic compounds by enzymes in cell extracts of the fungus Cunninghamella elegans.

Authors:  L P Wackett; D T Gibson
Journal:  Biochem J       Date:  1982-07-01       Impact factor: 3.857

7.  Crystal and molecular structure of the antibiotic blasticidin S hydrochloride pentahydrate.

Authors:  V Swaminathan; J L Smith; M Sundaralingam; C Coutsogeorgopoulos; G Kartha
Journal:  Biochim Biophys Acta       Date:  1981-10-27

8.  Isolation and characterization of mildiomycin, a new nucleoside antibiotic.

Authors:  S Harada; T Kishi
Journal:  J Antibiot (Tokyo)       Date:  1978-06       Impact factor: 2.649

9.  A kinetic mechanism for modulation of the activity of microsomal UDP-glucuronyltransferase by phospholipids. Effects of lysophosphatidylcholines.

Authors:  Y Hochman; D Zakim; D A Vessey
Journal:  J Biol Chem       Date:  1981-05-25       Impact factor: 5.157

10.  Nucleosides XXXII. On the Structure of blasticidin S, a nucleoside antibiotic.

Authors:  J J Fox; K A Watanabe
Journal:  Tetrahedron Lett       Date:  1966-03       Impact factor: 2.415
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  3 in total

1.  The Amipurimycin and Miharamycin Biosynthetic Gene Clusters: Unraveling the Origins of 2-Aminopurinyl Peptidyl Nucleoside Antibiotics.

Authors:  Anthony J Romo; Taro Shiraishi; Hideo Ikeuchi; Geng-Min Lin; Yujie Geng; Yu-Hsuan Lee; Priscilla H Liem; Tianlu Ma; Yasushi Ogasawara; Kazuo Shin-Ya; Makoto Nishiyama; Tomohisa Kuzuyama; Hung-Wen Liu
Journal:  J Am Chem Soc       Date:  2019-09-03       Impact factor: 15.419

2.  Identification of a novel glucuronyltransferase from Streptomyces chromofuscus ATCC 49982 for natural product glucuronidation.

Authors:  Jie Ren; Caleb Don Barton; Kathryn Eternity Sorenson; Jixun Zhan
Journal:  Appl Microbiol Biotechnol       Date:  2022-01-27       Impact factor: 4.813

3.  Streptomyces lividans blasticidin S deaminase and its application in engineering a blasticidin S-producing strain for ease of genetic manipulation.

Authors:  Li Li; Jun Wu; Zixin Deng; T Mark Zabriskie; Xinyi He
Journal:  Appl Environ Microbiol       Date:  2013-02-01       Impact factor: 4.792
  3 in total

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