Quantitation of subclasses of mononuclear cells in normal human blood by membrane receptor studies.

A technique for quantitating mononuclear cells expressing E, EA(IgG), EAC receptors, immunoglobulin carrying cells and phagocytic cells in normal human venous blood is described; normal values for each of these classes of mononuclear cell are established. The effects of different methods of cell preparation have been considered; these were found not to have a significant effect on the final values. In addition, we describe a second category of 'fluorescent non-capping' cells and show that these are numerically equivalent to cells which ingest neutral red.