Literature DB >> 8110836

Guanine nucleotide exchange factor for eukaryotic initiation factor-2. Cloning of cDNA for the delta-subunit of rabbit translation initiation factor-2B.

N T Price1, G Francia, L Hall, C G Proud.   

Abstract

Peptide sequence data for rabbit eIF-2B delta were obtained and used to design redundant oligonucleotides for PCR. RNA was isolated from rabbit liver and used to direct the synthesis of total cDNA. A rabbit eIF-2B delta transcript was then amplified by PCR and sequenced. The PCR product was used to isolate a clone from a rabbit liver cDNA library. RACE (rapid amplification of cDNA ends) was used to obtain further 5' sequence. Subsequently, a full length cDNA was obtained from a rabbit reticulocyte library. PCR was used to confirm that the sequence is the same for the liver factor. The sequence obtained shows strong homology to that of yeast eIF-2B delta, the GCD2 gene product.

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Year:  1994        PMID： 8110836     DOI： 10.1016/0167-4781(94)90037-x

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  6 in total

1.  Cloning and expression of the liver and muscle isoforms of ovine carnitine palmitoyltransferase 1: residues within the N-terminus of the muscle isoform influence the kinetic properties of the enzyme.

Authors:  Nigel T Price; Vicky N Jackson; Feike R van der Leij; Jacqueline M Cameron; Maureen T Travers; Beatrijs Bartelds; Nicolette C Huijkman; Victor A Zammit
Journal:  Biochem J       Date:  2003-06-15       Impact factor: 3.857

2.  eIF2B, the guanine nucleotide-exchange factor for eukaryotic initiation factor 2. Sequence conservation between the alpha, beta and delta subunits of eIF2B from mammals and yeast.

Authors:  N T Price; H Mellor; B L Craddock; K M Flowers; S R Kimball; T Wilmer; L S Jefferson; C G Proud
Journal:  Biochem J       Date:  1996-09-01       Impact factor: 3.857

3.  Cloning and expression of cDNAs for the beta subunit of eukaryotic initiation factor-2B, the guanine nucleotide exchange factor for eukaryotic initiation factor-2.

Authors:  B L Craddock; N T Price; C G Proud
Journal:  Biochem J       Date:  1995-08-01       Impact factor: 3.857

4.  Cloning of cDNA for the gamma-subunit of mammalian translation initiation factor 2B, the guanine nucleotide-exchange factor for eukaryotic initiation factor 2.

Authors:  N T Price; S R Kimball; L S Jefferson; C G Proud
Journal:  Biochem J       Date:  1996-09-01       Impact factor: 3.857

5.  Molecular cloning and characterization of cDNA encoding the alpha subunit of the rat protein synthesis initiation factor eIF-2B.

Authors:  K M Flowers; S R Kimball; R C Feldhoff; A G Hinnebusch; L S Jefferson
Journal:  Proc Natl Acad Sci U S A       Date:  1995-05-09       Impact factor: 11.205

6.  Mutations in GCD11, the structural gene for eIF-2 gamma in yeast, alter translational regulation of GCN4 and the selection of the start site for protein synthesis.

Authors:  D R Dorris; F L Erickson; E M Hannig
Journal:  EMBO J       Date:  1995-05-15       Impact factor: 11.598
  6 in total

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