Literature DB >> 8110188

Purification and characterization of phosphatidylinositol synthase from human placenta.

B E Antonsson1.   

Abstract

Phosphatidylinositol synthase (CDP-1,2-diacyl-sn-glycerol:myoinositol 3-phosphatidyltransferase, EC 2.7.8.11) was purified from the microsomal fraction of human placenta. The Triton X-100-extracted enzyme was purified 8300-fold over the microsomal fraction by affinity chromatography on CDP-diacylglycerol-Sepharose followed by ion-exchange chromatography on Mono Q. The purified enzyme had a molecular mass of 24,000 Da on SDS/PAGE. The enzyme had a pH optimum at 9.0, required Mn2+ or Mg2+, and was inhibited by Ca2+ and Zn2+. The Km for myo-inositol was determined to be 0.28 mM. Optimal activity was obtained at 0.2-0.4 mM CDP-diacylglycerol; higher concentrations of the lipid substrate inhibited the enzyme reaction. The enzyme was inhibited by nucleoside di- and tri-phosphates, Pi and PPi. CDP competitively inhibited the enzyme reaction with a Kis of 4 mM. The optimal temperature for the PtdIns synthase reaction was 50 degrees C.

Entities:  

Mesh：

Substances：

Year:  1994        PMID： 8110188      PMCID： PMC1137864          DOI： 10.1042/bj2970517

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  23 in total

1.  The enzymatic synthesis of inositol monophosphatide.

Authors:  H PAULUS; E P KENNEDY
Journal:  J Biol Chem       Date:  1960-05       Impact factor: 5.157

2.  Phosphatidylglycerol biosynthesis in Bacillus licheniformis Resolution of membrane-bound enzymes by affinity chromatography on cytidinediphospho-sn-1,2-diacylglycerol Sepharose.

Authors:  T J Larson; T Hirabayshi; W Dowhan
Journal:  Biochemistry       Date:  1976-03-09       Impact factor: 3.162

3.  On the solubility, stability and partial purification of CDPdiacyl-sn-glycerol: inositol transferase from rat brain.

Authors:  R H Rao; K P Strickland
Journal:  Biochim Biophys Acta       Date:  1974-05-29

4.  Membrane-associated phosphatidylglycerophosphate synthetase from Escherichia coli: purification by substrate affinity chromatography on cytidine 5'-diphospho-1,2-diacyl-sn-glycerol sepharose.

Authors:  T Hirabayashi; T J Larson; W Dowhan
Journal:  Biochemistry       Date:  1976-11-30       Impact factor: 3.162

5.  Phosphatidylinositol synthase from canine pancreas: solubilization by n-octyl glucopyranoside and stabilization by manganese.

Authors:  G S Parries; M Hokin-Neaverson
Journal:  Biochemistry       Date:  1984-09-25       Impact factor: 3.162

6.  Phosphatidylinositol biosynthesis in Saccharomyces cerevisiae: purification and properties of microsome-associated phosphatidylinositol synthase.

Authors:  A S Fischl; G M Carman
Journal:  J Bacteriol       Date:  1983-04       Impact factor: 3.490

7.  A simplification of the protein assay method of Lowry et al. which is more generally applicable.

Authors:  G L Peterson
Journal:  Anal Biochem       Date:  1977-12       Impact factor: 3.365

8.  Purification of phosphatidylinositol synthetase from rat brain by CDP-diacylglycerol affinity chromatography and properties of the purified enzyme.

Authors:  A Ghalayini; J Eichberg
Journal:  J Neurochem       Date:  1985-01       Impact factor: 5.372

9.  CDP-diglyceride:inositol transferase from rat liver. Purification and properties.

Authors:  T Takenawa; K Egawa
Journal:  J Biol Chem       Date:  1977-08-10       Impact factor: 5.157

10.  Effect of unsaturated fatty acids and Ca2+ on phosphatidylinositol synthesis and breakdown.

Authors:  T Takenawa; Y Nagai
Journal:  J Biochem       Date:  1982-03       Impact factor: 3.387
View more
  7 in total

1.  Cloning of Arabidopsis thaliana phosphatidylinositol synthase and functional expression in the yeast pis mutant.

Authors:  H W Xue; K Hosaka; G Plesch; B Mueller-Roeber
Journal:  Plant Mol Biol       Date:  2000-03       Impact factor: 4.076

2.  The phosphoinositide signaling cycle in myelin requires cooperative interaction with the axon.

Authors:  G Chakraborty; A Drivas; R Ledeen
Journal:  Neurochem Res       Date:  1999-02       Impact factor: 3.996

3.  Identification of rat liver phosphatidylinositol synthase as a 21 kDa protein.

Authors:  M E Monaco; M Feldman; D L Kleinberg
Journal:  Biochem J       Date:  1994-11-15       Impact factor: 3.857

4.  Time-dependent effects of lithium on the agonist-stimulated accumulation of second messenger inositol 1,4,5-trisphosphate in SH-SY5Y human neuroblastoma cells.

Authors:  G V Los; I P Artemenko; L E Hokin
Journal:  Biochem J       Date:  1995-10-01       Impact factor: 3.857

5.  Phosphatidylinositol synthesis is essential in bloodstream form Trypanosoma brucei.

Authors:  Kirstee L Martin; Terry K Smith
Journal:  Biochem J       Date:  2006-06-01       Impact factor: 3.857

6.  Structural basis for catalysis in a CDP-alcohol phosphotransferase.

Authors:  Giuliano Sciara; Oliver B Clarke; David Tomasek; Brian Kloss; Shantelle Tabuso; Rushelle Byfield; Raphael Cohn; Surajit Banerjee; Kanagalaghatta R Rajashankar; Vesna Slavkovic; Joseph H Graziano; Lawrence Shapiro; Filippo Mancia
Journal:  Nat Commun       Date:  2014-06-13       Impact factor: 14.919

Review 7.  Phosphoinositides, Major Actors in Membrane Trafficking and Lipid Signaling Pathways.

Authors:  Johan-Owen De Craene; Dimitri L Bertazzi; Séverine Bär; Sylvie Friant
Journal:  Int J Mol Sci       Date:  2017-03-15       Impact factor: 5.923
  7 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.