| Literature DB >> 8109750 |
S Ferraboli1, R Negri, E Di Mauro, S Barlati.
Abstract
A method for DNA sequencing that combines limited chemical degradation of 3'-fluorescent-labeled DNA with densitometric analysis using an automatic sequencer is presented. The DNA sequence is determined in a single electrophoretic lane by monitoring the intensities of bands representing products of cleavage at the four bases obtained by solvolysis in hot aqueous piperidine (10%) followed by treatment with hot formamide. An application of the method for the detection of point mutations is reported.Entities:
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Year: 1993 PMID： 8109750 DOI： 10.1006/abio.1993.1539
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365