Literature DB >> 8106079

High resolution visual mapping of stretched DNA by fluorescent hybridization.

I Parra1, B Windle.   

Abstract

We describe a method for stretching DNA, which, when combined with fluorescent hybridization procedures, forms a new mapping technology that produces a high resolution, vivid, multi-colour image and map. Restriction fragments and cosmid probes were successfully mapped by this procedure with validation by standard restriction mapping. A long range map of a > 200 kilobase region containing five copies of the amplified dihydrofolate reductase gene was easily generated within two days. This DNA mapping procedure offers a significant and rapid alternative to a variety of standard mapping procedures.

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Year:  1993        PMID： 8106079     DOI： 10.1038/ng0993-17

Source DB:  PubMed          Journal:  Nat Genet        ISSN： 1061-4036            Impact factor:   38.330


  61 in total

Review 1.  Imaging of single DNA molecule: applications to high-resolution genomic studies.

Authors:  J Herrick; A Bensimon
Journal:  Chromosome Res       Date:  1999       Impact factor: 5.239

2.  Quantifying single gene copy number by measuring fluorescent probe lengths on combed genomic DNA.

Authors:  J Herrick; X Michalet; C Conti; C Schurra; A Bensimon
Journal:  Proc Natl Acad Sci U S A       Date:  2000-01-04       Impact factor: 11.205

3.  Visualization of prekinetochore locus on the centromeric region of highly extended chromatin fibers: does kinetochore autoantigen CENP-C constitute a kinetochore organizing center?

Authors:  K Sugimoto; M Tsutsui; D AuCoin; B K Vig
Journal:  Chromosome Res       Date:  1999       Impact factor: 5.239

4.  Partially inverted tandem repeat isolated from pericentric region of chicken chromosome 8.

Authors:  Xiaofei Wang; Juan Li; Frederick C Leung
Journal:  Chromosome Res       Date:  2002       Impact factor: 5.239

5.  Shear-induced assembly of lambda-phage DNA.

Authors:  C Haber; D Wirtz
Journal:  Biophys J       Date:  2000-09       Impact factor: 4.033

6.  Autophosphorylation and ATM activation: additional sites add to the complexity.

Authors:  Sergei V Kozlov; Mark E Graham; Burkhard Jakob; Frank Tobias; Amanda W Kijas; Marcel Tanuji; Philip Chen; Phillip J Robinson; Gisela Taucher-Scholz; Keiji Suzuki; Sairai So; David Chen; Martin F Lavin
Journal:  J Biol Chem       Date:  2010-12-13       Impact factor: 5.157

7.  Single-step multicolor fluorescence in situ hybridization using semiconductor quantum dot-DNA conjugates.

Authors:  Laurent A Bentolila; Shimon Weiss
Journal:  Cell Biochem Biophys       Date:  2006       Impact factor: 2.194

8.  Duplication of a genomic region containing the Cdc2L1-2 and MMP21-22 genes on human chromosome 1p36.3 and their linkage to D1Z2.

Authors:  R Gururajan; J M Lahti; J Grenet; J Easton; I Gruber; P F Ambros; V J Kidd
Journal:  Genome Res       Date:  1998-09       Impact factor: 9.043

9.  Numbers and organization of RNA polymerases, nascent transcripts, and transcription units in HeLa nuclei.

Authors:  D A Jackson; F J Iborra; E M Manders; P R Cook
Journal:  Mol Biol Cell       Date:  1998-06       Impact factor: 4.138

10.  A fiber-FISH contig spanning the non-recombining region of the human Y chromosome.

Authors:  Susanne Röttger; Pauline H Yen; Werner Schempp
Journal:  Chromosome Res       Date:  2002       Impact factor: 5.239
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