Literature DB >> 8104771

CD4 T-lymphocyte determinations on whole blood specimens using a single-tube three-color assay.

J K Nicholson1, B M Jones, M Hubbard.   

Abstract

We evaluated a flow cytometric method for determining the proportion of CD4-positive T lymphocytes in whole blood using a single three-color tube containing fluorochrome-labeled CD45, CD3, and CD4. Various ways of gating this sample were evaluated and results were compared with data obtained in our standard six-tube, two-color assay gated on light scatter parameters. Excellent correlation was found between the three-color analysis using a CD45/side scatter gate and the standard two-color analysis with a light scatter gate. This single-tube three-color test is a promising assay for monitoring CD4+ T-lymphocytes.

Entities:  

Mesh：

Substances：

Year:  1993        PMID： 8104771     DOI： 10.1002/cyto.990140614

Source DB:  PubMed          Journal:  Cytometry        ISSN： 0196-4763


  10 in total

Review 1.  Adoption of single-platform technologies for enumeration of absolute T-lymphocyte subsets in peripheral blood.

Authors:  M R O'Gorman; J K Nicholson
Journal:  Clin Diagn Lab Immunol       Date:  2000-05

Review 2.  Monitoring patients with HIV disease.

Authors:  M Helbert; J Breuer
Journal:  J Clin Pathol       Date:  2000-04       Impact factor: 3.411

Review 3.  Immunophenotyping of bronchoalveolar lavage lymphocytes.

Authors:  R J Harbeck
Journal:  Clin Diagn Lab Immunol       Date:  1998-05

4.  Variables in the quantification of CD4 in normals and hairy cell leukemia patients.

Authors:  Lili Wang; Fatima Abbasi; Gregory A Jasper; Robert J Kreitman; David J Liewehr; Gerald E Marti; Maryalice Stetler-Stevenson
Journal:  Cytometry B Clin Cytom       Date:  2011-01       Impact factor: 3.058

5.  Contribution of CD4+, CD8+CD28+, and CD8+CD28- T cells to CD3+ lymphocyte homeostasis during the natural course of HIV-1 infection.

Authors:  A Caruso; S Licenziati; A D Canaris; A Cantalamessa; S Fiorentini; S Ausenda; D Ricotta; F Dima; F Malacarne; A Balsari; A Turano
Journal:  J Clin Invest       Date:  1998-01-01       Impact factor: 14.808

6.  Standardized method to minimize variability in a functional P2X(7) flow cytometric assay for a multi-center clinical trial.

Authors:  N L Korpi-Steiner; D Sheerar; E B Puffer; C Urben; J Boyd; A Guadarrama; K Schell; L C Denlinger
Journal:  Cytometry B Clin Cytom       Date:  2008-09       Impact factor: 3.058

Review 7.  CD4 immunophenotyping in HIV infection.

Authors:  David Barnett; Brooke Walker; Alan Landay; Thomas N Denny
Journal:  Nat Rev Microbiol       Date:  2008-11       Impact factor: 60.633

Review 8.  Current practices in laboratory monitoring of HIV infection.

Authors:  Madhu Vajpayee; Teena Mohan
Journal:  Indian J Med Res       Date:  2011-12       Impact factor: 2.375

9.  Small molecular fluorescence dyes for immuno cell analysis.

Authors:  Janine Jiang; Xue Li; Fei Mao; Xingyong Wu; Yong Chen
Journal:  Anal Biochem       Date:  2020-12-09       Impact factor: 3.365

10.  ProBDNF promotes sepsis-associated encephalopathy in mice by dampening the immune activity of meningeal CD4+ T cells.

Authors:  Ru-Yi Luo; Cong Luo; Feng Zhong; Wei-Yun Shen; Hui Li; Zhao-Lan Hu; Ru-Ping Dai
Journal:  J Neuroinflammation       Date:  2020-05-28       Impact factor: 8.322
  10 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.