| Literature DB >> 8102021 |
Abstract
The hemolytic action of palytoxin was exploited to develop a simple, sensitive assay with specificity based on a palytoxin neutralizing monoclonal antibody. Suspensions of murine erythrocytes incubated at 37 degrees C in round-bottom microtiter trays formed visible cell pellets which could be lysed by palytoxin. Hemolysis by palytoxin was time- and temperature-dependent, with a 24 hr detection limit of 1 pg/ml. The assay selectively detected palytoxin in a crude extract of Palythoa tuberculosa.Entities:
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Year: 1993 PMID： 8102021 DOI： 10.1016/0041-0101(93)90389-z
Source DB: PubMed Journal: Toxicon ISSN： 0041-0101 Impact factor: 3.033