Literature DB >> 810141

A simple method of characterizing mitochondrial ribonucleic acid.

D Haldar, B K Waters, D B Freeman.   

Abstract

A simple method of isolating and characterizing RNA from L-cell mitochondria is described. The mitochondrial fraction is lysed by sodium dodecyl sulphate, and the RNA fractionated by sucrose-density-gradient centrifugation. The efficacy of proteinase K in preventing ribonuclease activity is also demonstrated.

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Year:  1975        PMID： 810141      PMCID： PMC1165492          DOI： 10.1042/bj1470621

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  14 in total

1.  Determination of free amino groups in proteins by trinitrobenzenesulfonic acid.

Authors:  A F Habeeb
Journal:  Anal Biochem       Date:  1966-03       Impact factor: 3.365

2.  Mitochondrial ribonucleic acid from cultured animal cells. Comparison of pulse-labeled with steady state-labeled ribonucleic acid.

Authors:  D T Dubin
Journal:  J Biol Chem       Date:  1972-05-10       Impact factor: 5.157

3.  Mitochondrial RNA from cultured animal cells. Distinctive high-molecular-weight and 4 s species.

Authors:  D T Dubin; B S Montenecourt
Journal:  J Mol Biol       Date:  1970-03-14       Impact factor: 5.469

4.  Physicochemical characteristics of isolated 55-S mitochondrial ribosomes from rat-liver.

Authors:  H de Vries; R van d Koogh-Schuuring
Journal:  Biochem Biophys Res Commun       Date:  1973-09-05       Impact factor: 3.575

5.  Expression of the mitochondrial genome in HeLa cells. XIX. Occurrence in mitochondria of polyadenylic acid sequences, "free" and covalently linked to mitochondrial DNA-coded RNA.

Authors:  D Ojala; G Attardi
Journal:  J Mol Biol       Date:  1974-01-15       Impact factor: 5.469

6.  A new method using 'proteinase K' to prevent mRNA degradation during isolation from HeLa cells.

Authors:  U Wiegers; H Hilz
Journal:  Biochem Biophys Res Commun       Date:  1971-07-16       Impact factor: 3.575

7.  Expression of the mitochondrial genome in HeLa cells. I. Properties of the discrete RNA components from the mitochondrial fraction.

Authors:  B Attardi; G Attardi
Journal:  J Mol Biol       Date:  1971-01-28       Impact factor: 5.469

8.  Characterization of macromolecules by constant velocity sedimentation.

Authors:  H Noll
Journal:  Nature       Date:  1967-07-22       Impact factor: 49.962

9.  RNA metabolism in the HeLa cell nucleus.

Authors:  S Penman
Journal:  J Mol Biol       Date:  1966-05       Impact factor: 5.469

10.  Synthesis of ribonucleic acid by isolated rat liver mitochondria.

Authors:  S Fukamachi; B Bartoov; K B Freeman
Journal:  Biochem J       Date:  1972-06       Impact factor: 3.857
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