Literature DB >> 810088

Liquid nitrogen cryo-impacting: a new concept for cell disruption.

R A Smucker, R M Pfister.   

Abstract

High-efficiency disruption of bacteria can be accomplished in 2 or more min by the new procedure of liquid nitrogen cryo-impacting. Release of the dipicolinic acid-Ca2+ chelate paralleled the breakage of Bacillus megaterium endospores. Lactate dehydrogenase activity was much better in supernates from liquid nitrogen cryo-impacting-broken Escherichia coli cells than in those from sonically treated and broken E. coli cells.

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Year:  1975        PMID: 810088      PMCID: PMC187201          DOI: 10.1128/am.30.3.445-449.1975

Source DB:  PubMed          Journal:  Appl Microbiol        ISSN: 0003-6919


  4 in total

1.  Sonic disruption of spores of Bacillus cereus.

Authors:  J A BERGER; A G MARR
Journal:  J Gen Microbiol       Date:  1960-02

2.  Studies on lactic dehydrogenase of heart. I. Purity, kinetics, and equilibria.

Authors:  J B NEILANDS
Journal:  J Biol Chem       Date:  1952-11       Impact factor: 5.157

3.  Simple, inexpensive procedure for the disruption of bacteria.

Authors:  J M Ranhand
Journal:  Appl Microbiol       Date:  1974-07

4.  Electron microscopy of structural detail in frozen biological specimens.

Authors:  R L STEERE
Journal:  J Biophys Biochem Cytol       Date:  1957-01-25
  4 in total
  8 in total

1.  Isolation of a soluble component of Plasmodium berghei which induces immunity in rats.

Authors:  G D Grothaus; J P Kreier
Journal:  Infect Immun       Date:  1980-04       Impact factor: 3.441

2.  Chitinase determinants of Vibrio vulnificus: gene cloning and applications of a chitinase probe.

Authors:  A T Wortman; C C Somerville; R R Colwell
Journal:  Appl Environ Microbiol       Date:  1986-07       Impact factor: 4.792

3.  In vivo analysis of the functions of gamma-tubulin-complex proteins.

Authors:  Yi Xiong; Berl R Oakley
Journal:  J Cell Sci       Date:  2009-10-27       Impact factor: 5.285

4.  Acid-soluble spore proteins of Bacillus subtilis.

Authors:  W C Johnson; D J Tipper
Journal:  J Bacteriol       Date:  1981-06       Impact factor: 3.490

5.  A 3D-Fluorescence Fingerprinting Approach to Detect Physiological Modifications Induced by Pesticide Poisoning in Apis mellifera: A Preliminary Study.

Authors:  Christophe B Y Cordella; Alberto Izquierdo-Rodriguez; Marie-José Durand-Thouand
Journal:  J Fluoresc       Date:  2019-12-02       Impact factor: 2.217

6.  Transformation of Bacillus thuringiensis protoplasts by plasmid deoxyribonucleic acid.

Authors:  P A Martin; J R Lohr; D H Dean
Journal:  J Bacteriol       Date:  1981-02       Impact factor: 3.490

7.  Protein Complex Affinity Capture from Cryomilled Mammalian Cells.

Authors:  John LaCava; Hua Jiang; Michael P Rout
Journal:  J Vis Exp       Date:  2016-12-09       Impact factor: 1.355

8.  Set screw homogenization of murine ocular tissue, including the whole eye.

Authors:  Sarah W Gooding; Micah A Chrenek; Salma Ferdous; John M Nickerson; Jeffrey H Boatright
Journal:  Mol Vis       Date:  2018-10-26       Impact factor: 2.367

  8 in total

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