Literature DB >> 8098963

Repeated sequences as genetic markers in pooled tissue samples.

R P Hellens1, T H Ellis, D Lee, L Turner.   

Abstract

We show, using the PDR1 element of pea, that dispersed repeated sequences of moderate copy number can be used simply and efficiently to generate markers linked to a trait of interest. Inspection of hybridization patterns of repeated sequences to DNA mixtures of pooled genotypes is a sensitive way of detecting such markers. The large number of bands in tracks of digests of these mixtures allows the simultaneous sampling of loci at many places in the genome, and the many unlinked loci serve as internal controls. It is also shown that intensity ratios calculated from these band differences can be used to give a rough estimate of linkage distance.

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Year:  1993        PMID： 8098963     DOI： 10.1007/bf00039003

Source DB:  PubMed          Journal:  Plant Mol Biol        ISSN： 0167-4412            Impact factor:   4.076


  9 in total

1.  Extreme heterogeneity of Ty1-copia group retrotransposons in plants.

Authors:  A J Flavell; D B Smith; A Kumar
Journal:  Mol Gen Genet       Date:  1992-01

2.  Polymerase chain reaction based mapping of rye involving repeated DNA sequences.

Authors:  P M Rogowsky; K W Shepherd; P Langridge
Journal:  Genome       Date:  1992-08       Impact factor: 2.166

3.  Linkage maps in pea.

Authors:  T H Ellis; L Turner; R P Hellens; D Lee; C L Harker; C Enard; C Domoney; D R Davies
Journal:  Genetics       Date:  1992-03       Impact factor: 4.562

4.  Isolation of molecular markers from specific chromosomal intervals using DNA pools from existing mapping populations.

Authors:  J J Giovannoni; R A Wing; M W Ganal; S D Tanksley
Journal:  Nucleic Acids Res       Date:  1991-12-11       Impact factor: 16.971

5.  Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations.

Authors:  R W Michelmore; I Paran; R V Kesseli
Journal:  Proc Natl Acad Sci U S A       Date:  1991-11-01       Impact factor: 11.205

6.  Use of isogenic lines and simultaneous probing to identify DNA markers tightly linked to the tm-2a gene in tomato.

Authors:  N D Young; D Zamir; M W Ganal; S D Tanksley
Journal:  Genetics       Date:  1988-10       Impact factor: 4.562

7.  Inbreeding and Linkage.

Authors:  J B Haldane; C H Waddington
Journal:  Genetics       Date:  1931-07       Impact factor: 4.562

8.  A copia-like element in Pisum demonstrates the uses of dispersed repeated sequences in genetic analysis.

Authors:  D L Lee; T H Ellis; L Turner; R P Hellens; W G Cleary
Journal:  Plant Mol Biol       Date:  1990-11       Impact factor: 4.076

9.  Rapid identification of markers linked to a Pseudomonas resistance gene in tomato by using random primers and near-isogenic lines.

Authors:  G B Martin; J G Williams; S D Tanksley
Journal:  Proc Natl Acad Sci U S A       Date:  1991-03-15       Impact factor: 11.205
  9 in total
  5 in total

1.  Cloning and characterisation of a cytosolic glutathione reductase cDNA from pea (Pisum sativum L.) and its expression in response to stress.

Authors:  R G Stevens; G P Creissen; P M Mullineaux
Journal:  Plant Mol Biol       Date:  1997-11       Impact factor: 4.076

2.  A gene causing Hermansky-Pudlak syndrome in a Puerto Rican population maps to chromosome 10q2.

Authors:  S C Wildenberg; W S Oetting; C Almodóvar; M Krumwiede; J G White; R A King
Journal:  Am J Hum Genet       Date:  1995-10       Impact factor: 11.025

3.  Plastidial glyceraldehyde-3-phosphate dehydrogenase deficiency leads to altered root development and affects the sugar and amino acid balance in Arabidopsis.

Authors:  Jesús Muñoz-Bertomeu; Borja Cascales-Miñana; Jose Miguel Mulet; Edurne Baroja-Fernández; Javier Pozueta-Romero; Josef M Kuhn; Juan Segura; Roc Ros
Journal:  Plant Physiol       Date:  2009-08-12       Impact factor: 8.340

4.  A new family of dispersed repeats from Brassica nigra: characterization and localization.

Authors:  R Kapila; M S Negi; P This; M Delseny; P S Srivastava; M Lakshmikumaran
Journal:  Theor Appl Genet       Date:  1996-11       Impact factor: 5.699

5.  The plastidial glyceraldehyde-3-phosphate dehydrogenase is critical for viable pollen development in Arabidopsis.

Authors:  Jesús Muñoz-Bertomeu; Borja Cascales-Miñana; Asunción Irles-Segura; Isabel Mateu; Adriano Nunes-Nesi; Alisdair R Fernie; Juan Segura; Roc Ros
Journal:  Plant Physiol       Date:  2010-01-27       Impact factor: 8.340
  5 in total

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