Literature DB >> 8096487

Characterization of a lysine-to-glutamic acid mutation in a conservative sequence of farnesyl diphosphate synthase from Saccharomyces cerevisiae.

L Blanchard1, F Karst.   

Abstract

The mutant gene erg20-2 was isolated from a yeast strain defective in farnesyl diphosphate synthase (FPPS). This strain had the unusual property of excreting prenyl alcohols such as geraniol. The nucleotide (nt) sequence, compared with that of the wild-type gene, showed a single nt change, resulting in a Lys197-->Glu substitution in FPPS which is directly involved in terpenic alcohol formation. In addition, disruption of ERG20 revealed that in yeast no other prenyl transferase is able to synthesize the FPP molecules required for essential non-sterol metabolites.

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Year:  1993        PMID： 8096487     DOI： 10.1016/0378-1119(93)90326-x

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  17 in total

1.  Sterol metabolism.

Authors:  Pierre Benveniste
Journal:  Arabidopsis Book       Date:  2002-03-27

2.  Molecular characterization and expression analysis of a gene encoding for farnesyl diphosphate synthase from Euphorbia pekinensis Rupr.

Authors:  Xiaoying Cao; Ting Yin; Qian Miao; Changgen Li; Xiuyun Ju; Yong Sun; Jihong Jiang
Journal:  Mol Biol Rep       Date:  2011-05-26       Impact factor: 2.316

3.  Identification of a lysine residue important for the catalytic activity of yeast farnesyl diphosphate synthase.

Authors:  Marc J C Fischer; Sophie Meyer; Patricia Claudel; Marc Bergdoll; Francis Karst
Journal:  Protein J       Date:  2011-06       Impact factor: 2.371

4.  Isoprenyl diphosphate synthases: protein sequence comparisons, a phylogenetic tree, and predictions of secondary structure.

Authors:  A Chen; P A Kroon; C D Poulter
Journal:  Protein Sci       Date:  1994-04       Impact factor: 6.725

5.  Regulation of product chain length by isoprenyl diphosphate synthases.

Authors:  L C Tarshis; P J Proteau; B A Kellogg; J C Sacchettini; C D Poulter
Journal:  Proc Natl Acad Sci U S A       Date:  1996-12-24       Impact factor: 11.205

6.  The Saccharomyces cerevisiae mevalonate diphosphate decarboxylase is essential for viability, and a single Leu-to-Pro mutation in a conserved sequence leads to thermosensitivity.

Authors:  T Bergès; D Guyonnet; F Karst
Journal:  J Bacteriol       Date:  1997-08       Impact factor: 3.490

7.  Yeast farnesyl-diphosphate synthase: site-directed mutagenesis of residues in highly conserved prenyltransferase domains I and II.

Authors:  L Song; C D Poulter
Journal:  Proc Natl Acad Sci U S A       Date:  1994-04-12       Impact factor: 11.205

8.  Geranylgeranyl diphosphate synthase in fission yeast is a heteromer of farnesyl diphosphate synthase (FPS), Fps1, and an FPS-like protein, Spo9, essential for sporulation.

Authors:  Yanfang Ye; Makoto Fujii; Aiko Hirata; Makoto Kawamukai; Chikashi Shimoda; Taro Nakamura
Journal:  Mol Biol Cell       Date:  2007-06-27       Impact factor: 4.138

9.  Cloning of an Arabidopsis thaliana cDNA coding for farnesyl diphosphate synthase by functional complementation in yeast.

Authors:  D Delourme; F Lacroute; F Karst
Journal:  Plant Mol Biol       Date:  1994-12       Impact factor: 4.076

10.  New insights into molecular recognition of 1,1-bisphosphonic acids by farnesyl diphosphate synthase.

Authors:  Mariana Ferrer-Casal; Catherine Li; Melina Galizzi; Carlos A Stortz; Sergio H Szajnman; Roberto Docampo; Silvia N J Moreno; Juan B Rodriguez
Journal:  Bioorg Med Chem       Date:  2013-11-13       Impact factor: 3.641
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