OBJECTIVE: Our purpose was to assess the presence and frequency of gamma delta T cells in the decidua of term and first-trimester pregnancies. STUDY DESIGN: Term and first-trimester placentas were obtained from normal subjects. Frozen sections and cell suspensions were prepared from decidual tissue and stained with monoclonal antibodies to T cell markers. Cell sorter analysis was performed. RESULTS: gamma delta T cells in term decidual cell preparations were enriched 2.4-fold compared with peripheral blood. Immunohistochemical staining of term decidual tissue demonstrated many gamma delta + and CD3+ T cells, fewer CD8+ cells, and rare CD4+ cells. In contrast, first-trimester decidua was found to be devoid of gamma delta + T cells, by both cell sorter analysis and immunohistochemical methods. CONCLUSIONS: Term, but not early, decidua harbors a resident T-cell population that is significantly enriched in gamma delta T cells compared with blood. These lymphocytes may provide an added defense mechanism against infection during the peripartum.
OBJECTIVE: Our purpose was to assess the presence and frequency of gamma delta T cells in the decidua of term and first-trimester pregnancies. STUDY DESIGN: Term and first-trimester placentas were obtained from normal subjects. Frozen sections and cell suspensions were prepared from decidual tissue and stained with monoclonal antibodies to T cell markers. Cell sorter analysis was performed. RESULTS: gamma delta T cells in term decidual cell preparations were enriched 2.4-fold compared with peripheral blood. Immunohistochemical staining of term decidual tissue demonstrated many gamma delta + and CD3+ T cells, fewer CD8+ cells, and rare CD4+ cells. In contrast, first-trimester decidua was found to be devoid of gamma delta + T cells, by both cell sorter analysis and immunohistochemical methods. CONCLUSIONS: Term, but not early, decidua harbors a resident T-cell population that is significantly enriched in gamma delta T cells compared with blood. These lymphocytes may provide an added defense mechanism against infection during the peripartum.
Authors: Christine Waasdorp Hurtado; Lucy Golden-Mason; Megan Brocato; Mona Krull; Michael R Narkewicz; Hugo R Rosen Journal: PLoS One Date: 2010-08-30 Impact factor: 3.240
Authors: Ria Lassaunière; Alfred Musekiwa; Glenda E Gray; Louise Kuhn; Caroline T Tiemessen Journal: Retrovirology Date: 2016-06-10 Impact factor: 4.602