Literature DB >> 8094396

Use of monoclonal antibodies specific for the a determinant of K88 pili for detection of enterotoxigenic Escherichia coli in pigs.

R B Westerman1, G W Fortner, K W Mills, R M Phillips, J M Greenwood.   

Abstract

Monoclonal antibodies directed against the a determinant of K88 pili from porcine enterotoxigenic Escherichia coli which react with all three K88 variants have been produced. These antibodies have been used for diagnosis of porcine enterotoxigenic E. coli in a direct enzyme-linked immunosorbent assay with sensitivity to 50 ng of pilus protein per ml.

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Year:  1993        PMID： 8094396      PMCID： PMC262756          DOI： 10.1128/jcm.31.2.311-314.1993

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  25 in total

1.  Acetylornithinase of Escherichia coli: partial purification and some properties.

Authors:  H J VOGEL; D M BONNER
Journal:  J Biol Chem       Date:  1956-01       Impact factor: 5.157

2.  Peroxidase-labeled antibody. A new method of conjugation.

Authors:  P K Nakane; A Kawaoi
Journal:  J Histochem Cytochem       Date:  1974-12       Impact factor: 2.479

3.  A new E. coli serotype O149:K9 (B), K88ac (L): H10 isolated from diseased swine.

Authors:  I Orskov; F Orskov; W Wittig; E J Sweeney
Journal:  Acta Pathol Microbiol Scand       Date:  1969

4.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

5.  Colonial morphology of Escherichia coli on Tergitol-7 medium.

Authors:  R K Scherer
Journal:  Appl Microbiol       Date:  1966-03

6.  K99 surface antigen of Escherichia coli: purification and partial characterization.

Authors:  R E Isaacson
Journal:  Infect Immun       Date:  1977-01       Impact factor: 3.441

7.  K ANTIGENS K88AB(L) AND K88AC(L) IN E. COLI. A NEW O ANTIGEN: 0147 AND A NEW K ANTIGEN: K89(B).

Authors:  I ORSKOV; F ORSKOV; W J SOJKA; W WITTIG
Journal:  Acta Pathol Microbiol Scand       Date:  1964

8.  Episome-carried surface antigen K88 of Escherichia coli. II. Isolation and chemical analysis.

Authors:  S Stirm; F Orskov; I Orskov; B Mansa
Journal:  J Bacteriol       Date:  1967-02       Impact factor: 3.490

9.  Association of Escherichia coli with the small intestinal epithelium. I. Comparison of enteropathogenic and nonenteropathogenic porcine strains in pigs.

Authors:  H U Bertschinger; H W Moon; S C Whipp
Journal:  Infect Immun       Date:  1972-04       Impact factor: 3.441

10.  Immunity to Escherichia coli in pigs: adhesion of enteropathogenic Escherichia coli to isolated intestinal epithelial cells.

Authors:  M R Wilson; A W Hohmann
Journal:  Infect Immun       Date:  1974-10       Impact factor: 3.441
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  2 in total

1.  Production and characterization of monoclonal antibodies specific for the lipopolysaccharide of Escherichia coli O157.

Authors:  R B Westerman; Y He; J E Keen; E T Littledike; J Kwang
Journal:  J Clin Microbiol       Date:  1997-03       Impact factor: 5.948

2.  Production and characterization of monoclonal antibodies specific for lipooligosaccharide of Serpulina hyodysenteriae.

Authors:  R B Westerman; R M Phillips; L A Joens
Journal:  J Clin Microbiol       Date:  1995-08       Impact factor: 5.948
  2 in total

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