Literature DB >> 8089226

Purification of alpha-1-antitrypsin monomer by preparative electrophoresis.

F Spada1, G Candiano, C Sergi, G M Ghiggeri, F Callea, R Gusmano.   

Abstract

Alfa-1-antitrypsin (alpha 1AT) was purified by pseudoligand chromatography and preparative electrophoresis from the serum of a patient with alpha 1AT deficiency. The combination of the two techniques yielded a high grade batch of alpha 1AT monomer and this was successfully used to purify the protein from the serum of PiMIM1, PiMIM2, and PiZZ phenotype subjects. This procedure should facilitate structural studies of alpha 1AT variants susceptible to intracellular accumulation.

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Year:  1994        PMID： 8089226      PMCID： PMC502112          DOI： 10.1136/jcp.47.7.661

Source DB:  PubMed          Journal:  J Clin Pathol        ISSN： 0021-9746            Impact factor:   3.411


  9 in total

1.  Protein transport. Z and the insoluble answer.

Authors:  R N Sifers
Journal:  Nature       Date:  1992-06-18       Impact factor: 49.962

2.  The mechanism of Z alpha 1-antitrypsin accumulation in the liver.

Authors:  D A Lomas; D L Evans; J T Finch; R W Carrell
Journal:  Nature       Date:  1992-06-18       Impact factor: 49.962

3.  Characterization of alpha1-antitrypsin in the inclusion bodies from the liver in alpha 1-antitrypsin deficiency.

Authors:  J O Jeppsson; C Larsson; S Eriksson
Journal:  N Engl J Med       Date:  1975-09-18       Impact factor: 91.245

4.  Isolation, chemical, and physical properties of alpha-1-antitrypsin.

Authors:  P Musiani; T B Tomasi
Journal:  Biochemistry       Date:  1976-02-24       Impact factor: 3.162

5.  Purification of human alpha 1-antitrypsin by affinity chromatography on sepharose bound concanavalin A.

Authors:  R J Murthy; A Hercz
Journal:  FEBS Lett       Date:  1973-06-01       Impact factor: 4.124

6.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

7.  Immunohistochemical and genetic characterization of the M Cagliari alpha-1-antitrypsin molecule (M-like alpha-1-antitrypsin deficiency).

Authors:  C Sergi; G G Consalez; G Fabbretti; M Brisigotti; G Faa; V Costa; G Romeo; F Callea
Journal:  Lab Invest       Date:  1994-01       Impact factor: 5.662

8.  Kinetics of association of serine proteinases with native and oxidized alpha-1-proteinase inhibitor and alpha-1-antichymotrypsin.

Authors:  K Beatty; J Bieth; J Travis
Journal:  J Biol Chem       Date:  1980-05-10       Impact factor: 5.157

9.  Identification of PiZ gene products in liver tissue by a monoclonal antibody specific for the Z mutant of alpha 1-antitrypsin.

Authors:  F Callea; M Brisigotti; G Faa; L Lucini; S Eriksson
Journal:  J Hepatol       Date:  1991-05       Impact factor: 25.083
  9 in total

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