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Literature DB >> 8088542

Purification of the Escherichia coli integration host factor (IHF) in one chromatographic step.

Abstract

The integration host factor (IHF) participates in a diverse array of DNA transactions such as replication, recombination and gene expression. We describe a fast and very efficient isolation procedure which yields highly purified IHF in one chromatographic step.

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Year: 1994 PMID： 8088542 DOI： 10.1016/0378-1119(94)90056-6

Source DB: PubMed Journal: Gene ISSN： 0378-1119 Impact factor: 3.688

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Cited

7 in total

1. Energy-independent helicase activity of a viral genome packaging motor.

Authors: Jenny R Chang; Benjamin T Andrews; Carlos E Catalano
Journal: Biochemistry Date: 2011-12-30 Impact factor: 3.162

2. Mutational analysis of intervening sequences connecting the binding sites for integration host factor, PepA, PurR, and RNA polymerase in the control region of the Escherichia coli carAB operon, encoding carbamoylphosphate synthase.

Authors: Neel Devroede; Nadine Huysveld; Daniel Charlier
Journal: J Bacteriol Date: 2006-05 Impact factor: 3.490

3. TraY and integration host factor oriT binding sites and F conjugal transfer: sequence variations, but not altered spacing, are tolerated.

Authors: Sarah L Williams; Joel F Schildbach
Journal: J Bacteriol Date: 2007-03-09 Impact factor: 3.490

7. Integration of regulatory signals through involvement of multiple global regulators: control of the Escherichia coli gltBDF operon by Lrp, IHF, Crp, and ArgR.

Authors: Ligi Paul; Pankaj K Mishra; Robert M Blumenthal; Rowena G Matthews
Journal: BMC Microbiol Date: 2007-01-18 Impact factor: 3.605

7 in total

Purification of the Escherichia coli integration host factor (IHF) in one chromatographic step.

1. Energy-independent helicase activity of a viral genome packaging motor.

2. Mutational analysis of intervening sequences connecting the binding sites for integration host factor, PepA, PurR, and RNA polymerase in the control region of the Escherichia coli carAB operon, encoding carbamoylphosphate synthase.

3. TraY and integration host factor oriT binding sites and F conjugal transfer: sequence variations, but not altered spacing, are tolerated.

4. Molecular beacon-equilibrium cyclization detection of DNA-protein complexes.

5. Plasmid r1 conjugative DNA processing is regulated at the coupling protein interface.

6. Highly organized DnaA-oriC complexes recruit the single-stranded DNA for replication initiation.

7. Integration of regulatory signals through involvement of multiple global regulators: control of the Escherichia coli gltBDF operon by Lrp, IHF, Crp, and ArgR.