| Literature DB >> 8087110 |
A M Alves1, D F De Almeida, W M von Krüger.
Abstract
Reversible changes in kinetoplast DNA (kDNA) minicircles sequences were observed in clones of Trypanosoma cruzi strain Y, following a number of passages during exponential growth phase or after subcloning in blood-free medium. kDNA restriction patterns of clones were similar to those of the original uncloned strain, while subclones presented distinct kDNA restriction patterns. Homology experiments demonstrated strong hybridization between kDNA with the same electrophoretic mobility patterns while only weak signals were observed with kDNA of different patterns. The changes observed, which are unprecedented in T. cruzi clones, characterize transkinetoplastidy, and seem to be associated with similarly reversible changes both in zymodeme and in infectivity.Entities:
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Year: 1994 PMID： 8087110 DOI： 10.1111/j.1550-7408.1994.tb06099.x
Source DB: PubMed Journal: J Eukaryot Microbiol ISSN： 1066-5234 Impact factor: 3.346