| Literature DB >> 8086451 |
Abstract
A tRNA operon (trnA) from Lactococcus lactis consisting of seven tRNA genes and a 5S rRNA gene was cloned and sequenced. Promoter-fusion of the trnA promoter to a promoter-less beta-galactosidase gene of Leuconostoc mesenteroides resulted in high levels of beta-galactosidase activity in L. lactis. Searching for sequences with similarity to the sequence of the promoter region revealed a consensus sequence of promoters preceeding rRNA operons and tRNA operons from Lactococcus species including a not previously described conserved sequence (AGTT).Entities:
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Year: 1994 PMID: 8086451 DOI: 10.1016/0167-4781(94)90256-9
Source DB: PubMed Journal: Biochim Biophys Acta ISSN: 0006-3002