| Literature DB >> 8086312 |
H T Cook1, C Avey, G M Taylor.
Abstract
To investigate the role of interleukin-1 beta (IL-1 beta) in glomerulonephritis we studied the presence of IL-1 beta by in-situ hybridization in in-situ immune complex glomerulonephritis in the rat. Glomerulonephritis was induced in preimmunized rats by unilateral renal perfusion with cationized human IgG. In-situ hybridization was performed on frozen sections with a battery of four 30mer oligonucleotide DNA probes 3' end labelled with 35S-dATP. IL-1 beta mRNA was detectable in nephritic glomeruli at 6 and 24 hours after induction of glomerulonephritis. Signal was maximal at 48 hours and markedly reduced by 4 days. The peak of IL-1 beta transcription coincided with the major monocyte influx into glomeruli consistent with a role for IL-1 beta as a mediator of glomerular hypercellularity.Entities:
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Year: 1994 PMID： 8086312 PMCID： PMC2001800
Source DB: PubMed Journal: Int J Exp Pathol ISSN： 0959-9673 Impact factor: 1.925