Literature DB >> 8083527

Determination of arginase activity in macrophages: a micromethod.

I M Corraliza1, M L Campo, G Soler, M Modolell.   

Abstract

We propose a modification of Schimke's method for urea determination as a valuable micromethod for measuring arginase in activated macrophages. The method exhibits the following advantages: (a) it uses small amounts of samples (approximately 25,000 macrophages per assay); (b) it does not interfere with other related metabolites that are also present in the activated macrophage such as citrulline or arginine; (c) saturating concentrations of the substrate arginine can be used; and (d) it is much more sensitive than Schimke's method and can detect small amounts of urea, in the order of 0.02 mumol.

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Year:  1994        PMID： 8083527     DOI： 10.1016/0022-1759(94)90027-2

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  171 in total

1.  Endothelial arginase II responds to pharmacological inhibition by elevation in protein level.

Authors:  Karina Krotova; Jawaharlal M Patel; Edward R Block; Sergey Zharikov
Journal:  Mol Cell Biochem       Date:  2010-06-19       Impact factor: 3.396

2.  Characterization of the nature of granulocytic myeloid-derived suppressor cells in tumor-bearing mice.

Authors:  Je-In Youn; Michelle Collazo; Irina N Shalova; Subhra K Biswas; Dmitry I Gabrilovich
Journal:  J Leukoc Biol       Date:  2011-09-27       Impact factor: 4.962

3.  Hypoxia-inducible factor 2alpha regulates macrophage function in mouse models of acute and tumor inflammation.

Authors:  Hongxia Z Imtiyaz; Emily P Williams; Michele M Hickey; Shetal A Patel; Amy C Durham; Li-Jun Yuan; Rachel Hammond; Phyllis A Gimotty; Brian Keith; M Celeste Simon
Journal:  J Clin Invest       Date:  2010-07-19       Impact factor: 14.808

4.  Alterations in P-Glycoprotein Expression and Function Between Macrophage Subsets.

Authors:  Theodore J Cory; Hui He; Lee C Winchester; Santosh Kumar; Courtney V Fletcher
Journal:  Pharm Res       Date:  2016-07-18       Impact factor: 4.200

5.  Inhibition of endogenous activated protein C attenuates experimental autoimmune encephalomyelitis by inducing myeloid-derived suppressor cells.

Authors:  Leah M Alabanza; Naomi L Esmon; Charles T Esmon; Margaret S Bynoe
Journal:  J Immunol       Date:  2013-08-30       Impact factor: 5.422

6.  Vascular Dysfunction in Pneumocystis-Associated Pulmonary Hypertension Is Related to Endothelin Response and Adrenomedullin Concentration.

Authors:  Dan W Siemsen; Erin Dobrinen; Soo Han; Kari Chiocchi; Nicole Meissner; Steve D Swain
Journal:  Am J Pathol       Date:  2015-12-11       Impact factor: 4.307

7.  Arginase II inhibition prevents nitrate tolerance.

Authors:  S M L Khong; K L Andrews; N N Huynh; K Venardos; A Aprico; D L Michell; M Zarei; K T Moe; G J Dusting; D M Kaye; J P F Chin-Dusting
Journal:  Br J Pharmacol       Date:  2012-08       Impact factor: 8.739

8.  Polyamine-blocking therapy reverses immunosuppression in the tumor microenvironment.

Authors:  Candace S Hayes; Allyson C Shicora; Martin P Keough; Adam E Snook; Mark R Burns; Susan K Gilmour
Journal:  Cancer Immunol Res       Date:  2013-10-07       Impact factor: 11.151

9.  Methionine and methionine sulfoxide treatment induces M1/classical macrophage polarization and modulates oxidative stress and purinergic signaling parameters.

Authors:  Lien M Dos Santos; Tatiane M da Silva; Juliana H Azambuja; Priscila T Ramos; Pathise S Oliveira; Elita F da Silveira; Nathalia S Pedra; Kennia Galdino; Carlus A T do Couto; Mayara S P Soares; Rejane G Tavares; Roselia M Spanevello; Francieli M Stefanello; Elizandra Braganhol
Journal:  Mol Cell Biochem       Date:  2016-10-17       Impact factor: 3.396

10.  Arginase blockade protects against hepatic damage in warm ischemia-reperfusion.

Authors:  Geetha Jeyabalan; John R Klune; Atsunori Nakao; Nicole Martik; Guoyao Wu; Allan Tsung; David A Geller
Journal:  Nitric Oxide       Date:  2008-04-14       Impact factor: 4.427
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