Literature DB >> 8082195

The Escherichia coli recA gene increases UV-induced mitotic gene conversion in Saccharomyces cerevisiae.

V Vlcková1, L Cernáková, E Farkasová, J Brozmanová.   

Abstract

The effect of the Escherichia coli RecA protein on mitotic recombination in the diploid D7 strain of Saccharomyces cerevisiae damaged by UV radiation was investigated. The D7 strain was transformed by two modified versions of the pNF2 plasmid: one, containing the ADH-1 promoter, and the other containing the recA gene tandemly arranged behind the ADH-1 promoter region. Immunological analysis proved the presence of the 38-kDa RecA protein in D7/pNF2ADHrecA transformants. We observed a positive effect of recA gene expression on mitotic gene conversion, mainly at higher doses of UV radiation. The results indicate that a RecA-like activity could participate in steps preceding mitotic conversion events in yeast.

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Year:  1994        PMID： 8082195     DOI： 10.1007/bf00351789

Source DB:  PubMed          Journal:  Curr Genet        ISSN： 0172-8083            Impact factor:   3.886


  15 in total

1.  Yeast intrachromosomal recombination: long gene conversion tracts are preferentially associated with reciprocal exchange and require the RAD1 and RAD3 gene products.

Authors:  A Aguilera; H L Klein
Journal:  Genetics       Date:  1989-12       Impact factor: 4.562

Review 2.  The RecA protein as a recombinational repair system.

Authors:  M M Cox
Journal:  Mol Microbiol       Date:  1991-06       Impact factor: 3.501

3.  DMC1: a meiosis-specific yeast homolog of E. coli recA required for recombination, synaptonemal complex formation, and cell cycle progression.

Authors:  D K Bishop; D Park; L Xu; N Kleckner
Journal:  Cell       Date:  1992-05-01       Impact factor: 41.582

4.  Rad51 protein involved in repair and recombination in S. cerevisiae is a RecA-like protein.

Authors:  A Shinohara; H Ogawa; T Ogawa
Journal:  Cell       Date:  1992-05-01       Impact factor: 41.582

5.  Role of RecA protein spiral filaments in genetic recombination.

Authors:  P Howard-Flanders; S C West; A Stasiak
Journal:  Nature       Date:  1984 May 17-23       Impact factor: 49.962

6.  The PSO4 gene is responsible for an error-prone recombinational DNA repair pathway in Saccharomyces cerevisiae.

Authors:  H H de Andrade; E K Marques; A C Schenberg; J A Henriques
Journal:  Mol Gen Genet       Date:  1989-06

7.  Semidominant suppressors of Srs2 helicase mutations of Saccharomyces cerevisiae map in the RAD51 gene, whose sequence predicts a protein with similarities to procaryotic RecA proteins.

Authors:  A Aboussekhra; R Chanet; A Adjiri; F Fabre
Journal:  Mol Cell Biol       Date:  1992-07       Impact factor: 4.272

8.  The RAD52 gene is required for homothallic interconversion of mating types and spontaneous mitotic recombination in yeast.

Authors:  R E Malone; R E Esposito
Journal:  Proc Natl Acad Sci U S A       Date:  1980-01       Impact factor: 11.205

9.  The Escherichia coli recA gene increases resistance of the yeast Saccharomyces cerevisiae to ionizing and ultraviolet radiation.

Authors:  J Brozmanová; L Cernáková; V Vlcková; J Duraj; I Fridrichová
Journal:  Mol Gen Genet       Date:  1991-07

10.  Unusual stability of recombination intermediates made by Escherichia coli RecA protein.

Authors:  B Müller; I Burdett; S C West
Journal:  EMBO J       Date:  1992-07       Impact factor: 11.598
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  2 in total

Review 1.  How heterologously expressed Escherichia coli genes contribute to understanding DNA repair processes in Saccharomyces cerevisiae.

Authors:  Jela Brozmanová; Viera Vlcková; Miroslav Chovanec
Journal:  Curr Genet       Date:  2004-11-13       Impact factor: 3.886

Review 2.  Chlamydomonas reinhardtii: a convenient model system for the study of DNA repair in photoautotrophic eukaryotes.

Authors:  Daniel Vlcek; Andrea Sevcovicová; Barbara Sviezená; Eliska Gálová; Eva Miadoková
Journal:  Curr Genet       Date:  2007-11-09       Impact factor: 3.886
  2 in total

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