Literature DB >> 8080076

Analysis of uracil in DNA by gas chromatography-mass spectrometry.

B C Blount1, B N Ames.   

Abstract

A sensitive method using gas chromatography and negative chemical ionization mass spectrometry for the detection of uracil in DNA is described. Uracil DNA glycosylase is used to specifically cleave uracil from DNA. Once removed, uracil is derivatized with 3,5-bis(trifluoromethyl)benzyl bromide and the sample components are separated with gas chromatography. Negative chemical ionization mass spectrometry is used to quantitatively detect as little as 1 pg of uracil per 100 micrograms DNA. This assay is 10 times more sensitive than previously described quantitative methods for the detection of uracil in DNA. We apply this method as part of a project to determine why folic acid deficiency causes DNA breaks. We demonstrate that inhibition of folic acid metabolism induces an accumulation of uracil in DNA.

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Year:  1994        PMID： 8080076     DOI： 10.1006/abio.1994.1257

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  17 in total

1.  A method for detecting abasic sites in living cells: age-dependent changes in base excision repair.

Authors:  H Atamna; I Cheung; B N Ames
Journal:  Proc Natl Acad Sci U S A       Date:  2000-01-18       Impact factor: 11.205

2.  Detection of uracil within DNA using a sensitive labeling method for in vitro and cellular applications.

Authors:  Gergely Róna; Ildikó Scheer; Kinga Nagy; Hajnalka L Pálinkás; Gergely Tihanyi; Máté Borsos; Angéla Békési; Beáta G Vértessy
Journal:  Nucleic Acids Res       Date:  2015-10-01       Impact factor: 16.971

Review 3.  Opposing roles of folate in prostate cancer.

Authors:  Kevin J Rycyna; Dean J Bacich; Denise S O'Keefe
Journal:  Urology       Date:  2013-08-28       Impact factor: 2.649

4.  Polymorphisms in uracil-processing genes, but not one-carbon nutrients, are associated with altered DNA uracil concentrations in an urban Puerto Rican population.

Authors:  Aurelie Chanson; Laurence D Parnell; Eric D Ciappio; Zhenhua Liu; Jimmy W Crott; Katherine L Tucker; Joel B Mason
Journal:  Am J Clin Nutr       Date:  2009-04-29       Impact factor: 7.045

Review 5.  Folate and cancer: how DNA damage, repair and methylation impact on colon carcinogenesis.

Authors:  Susan J Duthie
Journal:  J Inherit Metab Dis       Date:  2010-06-11       Impact factor: 4.982

Review 6.  Damage to DNA by reactive oxygen and nitrogen species: role in inflammatory disease and progression to cancer.

Authors:  H Wiseman; B Halliwell
Journal:  Biochem J       Date:  1996-01-01       Impact factor: 3.857

7.  A one-step method for quantitative determination of uracil in DNA by real-time PCR.

Authors:  András Horváth; Beáta G Vértessy
Journal:  Nucleic Acids Res       Date:  2010-09-22       Impact factor: 16.971

8.  Uracil misincorporation into DNA and folic acid supplementation.

Authors:  Aditi Hazra; Jacob Selhub; Wei-Hsun Chao; Per Magne Ueland; David J Hunter; John A Baron
Journal:  Am J Clin Nutr       Date:  2009-11-18       Impact factor: 7.045

9.  An assay for uracil in human DNA at baseline: effect of marginal vitamin B6 deficiency.

Authors:  Susan T Mashiyama; Christine M Hansen; Esther Roitman; Stella Sarmiento; James E Leklem; Terry D Shultz; Bruce N Ames
Journal:  Anal Biochem       Date:  2007-09-01       Impact factor: 3.365

10.  Associations between single nucleotide polymorphisms in folate uptake and metabolizing genes with blood folate, homocysteine, and DNA uracil concentrations.

Authors:  Lauren DeVos; Aurelie Chanson; Zhenhua Liu; Eric D Ciappio; Laurence D Parnell; Joel B Mason; Katherine L Tucker; Jimmy W Crott
Journal:  Am J Clin Nutr       Date:  2008-10       Impact factor: 7.045
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