| Literature DB >> 8075522 |
F D Carvalho1, F Remião, P Vale, J A Timbrell, M L Bastos, M A Ferreira.
Abstract
An HPLC method using a glassy carbon working detector for measuring cysteine and reduced glutathione (GSH) in biological samples was developed. Oxidized glutathione (GSSG) was also measured after reduction with glutathione reductase. This study was conducted in human plasma. Cysteine and GSH standard curves were linear in the physiological range, presenting detection limits of 22.0 pmol and 6.0 pmol respectively. Plasmatic results found were 43.92 +/- 4.15, 4.50 +/- 0.65, and 0.19 +/- 0.12 mM (means +/- SE), for cysteine, GSH and GSSG, respectively. Peak specificity for cysteine and reduced glutathione was certified by their disappearance after treatment with N-ethylmaleimide.Entities:
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Year: 1994 PMID： 8075522 DOI： 10.1002/bmc.1130080308
Source DB: PubMed Journal: Biomed Chromatogr ISSN： 0269-3879 Impact factor: 1.902