Literature DB >> 8073635

Baculovirus expression of the nucleocapsid protein of a Puumala serotype Hantavirus.

C Schuldt1, L Zöller, E K Bautz, G Darai.   

Abstract

Recombinant baculoviruses were generated harboring the entire coding region of the S segment cDNA of Hantavirus strain CG 18-20 that belongs to the Puumala serotype. The recombinant nucleocapsid protein was expressed in Sf9 cells and shown to be antigenically identical with the authentic viral nucleocapsid protein by means of immunoblot analysis. Acute-phase and convalescent sera from European HFRS patients recognized the recombinant nucleocapsid protein in Western blots and the recombinant Baculovirus in indirect immunofluorescence assays. Insect cells infected with the recombinant Baculoviruses proved to be a suitable noninfectious substitute for Hantavirus-infected Vero E6 cells as an antigen source for immunodiagnostic assays allowing the detection of antibodies in HFRS patients.

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Year:  1994        PMID： 8073635     DOI： 10.1007/bf01703071

Source DB:  PubMed          Journal:  Virus Genes        ISSN： 0920-8569            Impact factor:   2.332


  25 in total

1.  Serum antibodies to structural proteins of Hantavirus arise at different times after infection.

Authors:  J Groen; J Dalrymple; S Fisher-Hoch; J G Jordans; J P Clement; A D Osterhaus
Journal:  J Med Virol       Date:  1992-08       Impact factor: 2.327

2.  Comparison of the deduced gene products of the L, M and S genome segments of hantaviruses.

Authors:  D Antic; C Y Kang; K Spik; C Schmaljohn; O Vapalahti; A Vaheri
Journal:  Virus Res       Date:  1992-06       Impact factor: 3.303

3.  Molecular characterization of the RNA S segment of nephropathia epidemica virus strain Hällnäs B1.

Authors:  R Stohwasser; L B Giebel; L Zöller; E K Bautz; G Darai
Journal:  Virology       Date:  1990-01       Impact factor: 3.616

4.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

5.  Isolation of the nephropathia epidemica agent in Sweden.

Authors:  B Niklasson; J Le Duc
Journal:  Lancet       Date:  1984-05-05       Impact factor: 79.321

6.  An efficient procedure for the isolation of recombinant baculovirus.

Authors:  J Pen; G W Welling; S Welling-Wester
Journal:  Nucleic Acids Res       Date:  1989-01-11       Impact factor: 16.971

7.  A novel mu-capture enzyme-linked immunosorbent assay based on recombinant proteins for sensitive and specific diagnosis of hemorrhagic fever with renal syndrome.

Authors:  L G Zöller; S Yang; P Gött; E K Bautz; G Darai
Journal:  J Clin Microbiol       Date:  1993-05       Impact factor: 5.948

8.  Supercoil sequencing: a fast and simple method for sequencing plasmid DNA.

Authors:  E Y Chen; P H Seeburg
Journal:  DNA       Date:  1985-04

9.  Coding strategy of the S genome segment of Hantaan virus.

Authors:  C S Schmaljohn; G B Jennings; J Hay; J M Dalrymple
Journal:  Virology       Date:  1986-12       Impact factor: 3.616

10.  Epidemic hemorrhagic fever with renal syndrome in Yugoslavia, 1986.

Authors:  A Gligić; M Obradović; R Stojanović; N Vujosević; A Ovcarić; M Frusic; C J Gibbs; C H Calisher; D C Gajdusek
Journal:  Am J Trop Med Hyg       Date:  1989-07       Impact factor: 2.345
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  3 in total

1.  Expression of the nucleoprotein of the Puumala virus from the recombinant Semliki Forest virus replicon: characterization and use as a potential diagnostic tool.

Authors:  A Billecocq; D Coudrier; F Boué; B Combes; H Zeller; M Artois; M Bouloy
Journal:  Clin Diagn Lab Immunol       Date:  2003-07

2.  Stable expression of nucleocapsid proteins of Puumala and Hantaan virus in mammalian cells.

Authors:  T M Welzel; R Kehm; C A Tidona; W Muranyi; G Darai
Journal:  Virus Genes       Date:  1998       Impact factor: 2.332

3.  Expression of immunogenic Puumala virus nucleocapsid protein in transgenic tobacco and potato plants.

Authors:  R Kehm; N J Jakob; T M Welzel; E Tobiasch; O Viczian; S Jock; K Geider; S Süle; G Darai
Journal:  Virus Genes       Date:  2001-01       Impact factor: 2.198
  3 in total

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