[The binding of estradiol to human polynuclear eosinophilic leukocytes].

High affinity estradiol binding is found in the fraction of human eosinophil rich, polynuclear leukocytes sedimenting between 800 times 10 g times mn and 24,000 times 20 g times mn. The apparent dissociation constant at equilibrium is approximately 0.6 nM, and the total number of binding sites per blood eosinophil leukocyte is 7,400 sites per cell. Hydrogen peroxyde (1 muM) enhances estradiol binding, a finding similar to that previously observed autoradiographically for the binding of estradiol to the eosinophils of rat uterus.