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Literature DB >> 807302

Analysis of developing enamel of the rat III. Carbohydrate, DEAE-sephadex, and immunological studies.

Abstract

Carbohydrate analyses showed L-fucose, D-xylose, D-mannose, D-arabinose, N-acetyl glucosamine, N-acetyl galactosamine, N-acetyl mannosamine, glucuronic acid and several unknown species to be present in the immature enamel or in one of its fractions. The concentration of carbohydrates was greatest in the water-extractable fraction and next greatest in the water-insoluble fraction. The water-extractable, EDTA-water-soluble, and water-insoluble fractions each differed in their spectrum of carbohydrates. With DEAE-Sephadex fractionation at pH 5.5, the water-extractable fraction was separated partially into 7 peaks. Four fractions were recovered from the 7 peaks, each of which differed from the others in amino acid and carbohydrate content and in polyacrylamide gel characteristics. Immunoelectrophoretic procedures showed that antibodies will develop against the EDTA-water-soluble fraction.

Entities: Chemical Species

Mesh：

Substances：

Year: 1975 PMID： 807302 DOI： 10.1007/BF02546605

Source DB: PubMed Journal: Calcif Tissue Res ISSN： 0008-0594

14 in total

1. Studies on fluorescent antibody staining II. Inhibition by sub- optimally conjugated antibody globulins.

Authors: G GOLDSTEIN; B H SPALDING; W B HUNT
Journal: Proc Soc Exp Biol Med Date: 1962-11

2. The localization of polysaccharides in developing teeth.

Authors: G BEVELANDER; P L JOHNSON
Journal: J Dent Res Date: 1955-02 Impact factor: 6.116

3. Organic constituents of enamel.

Authors: M V STACK
Journal: J Am Dent Assoc Date: 1954-03 Impact factor: 3.634

4. The Sephadex gel filtration characteristics of the neutral soluble proteins of embryonic bovine enamel.

Authors: G L Mechanic; E P Katz; M J Glimcher
Journal: Biochim Biophys Acta Date: 1967-01-18

5. Hexosamine and acid glycosaminoglycans in human teeth.

Authors: R D Clark; J G Smith; E A Davidson
Journal: Biochim Biophys Acta Date: 1965-11-01

Review 6. Chemical aspects of the matrix concept in calcified tissue organisation.

Authors: J E Eastoe
Journal: Calcif Tissue Res Date: 1968-07-15

7. Determination of neutral sugars in glycoproteins by gas-liquid chromatography.

Authors: W F Lehnhardt; R J Winzler
Journal: J Chromatogr Date: 1968-05-07

8. Electrophoretic and Sephadex gel filtration studies of bovine foetal enamel matrix at acid pH.

Authors: A G Fincham
Journal: Calcif Tissue Res Date: 1968-12-18

9. The ultracentrifugal and free zone electrophoretic characterization of the neutral soluble proteins of embryonic bovine enamel.

Authors: E P Katz; G L Mechanic; M J Glimcher
Journal: Biochim Biophys Acta Date: 1965-10-18

Analysis of developing enamel of the rat III. Carbohydrate, DEAE-sephadex, and immunological studies.

1. Studies on fluorescent antibody staining II. Inhibition by sub- optimally conjugated antibody globulins.

2. The localization of polysaccharides in developing teeth.

3. Organic constituents of enamel.

4. The Sephadex gel filtration characteristics of the neutral soluble proteins of embryonic bovine enamel.

5. Hexosamine and acid glycosaminoglycans in human teeth.

Review 6. Chemical aspects of the matrix concept in calcified tissue organisation.

7. Determination of neutral sugars in glycoproteins by gas-liquid chromatography.

8. Electrophoretic and Sephadex gel filtration studies of bovine foetal enamel matrix at acid pH.

9. The ultracentrifugal and free zone electrophoretic characterization of the neutral soluble proteins of embryonic bovine enamel.

10. Analysis of developing enamel of the rat. II. Electrophoretic and amino acid studies.

1. Identification of four extracellular-matrix enamel proteins during embryonic-rabbit tooth-organ development.

2. Sulfated glycoconjugates in rat incisor secretory ameloblasts and developing enamel matrix.

3. In vitro and in vivo autoimmune response to enamel matrix proteins.

4. The carbohydrate moiety of mineral-bound proteins from fetal enamel: a basis for enamelins heterogeneity.

5. Phosphophoryns-major noncollagenous proteins of rat incisor dentin.