| Literature DB >> 8071418 |
H Wang1, M Chen, Z N Berneman, G Delgado, J A DiPaolo.
Abstract
The polymerase chain reaction (PCR) was used to detect human herpesvirus-6 (HHV-6) DNA sequences from paraffin-embedded tissue from cervical cancer patients. Two of eight cases were positive for HHV-6 using two sets of HHV-6 primers. Hybridization of PCR products with specific radioisotope-labeled oligonucleotide probes confirmed the results. Furthermore, HHV-6 typing was possible by adapting restriction endonuclease digestion of PCR product. This method is useful for retrospective studies in investigating the etiologic role of HHV-6 in the development of human diseases.Entities:
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Year: 1994 PMID： 8071418 DOI： 10.1016/0166-0934(94)90026-4
Source DB: PubMed Journal: J Virol Methods ISSN： 0166-0934 Impact factor: 2.014