Literature DB >> 8071416

Detection of porcine reproductive respiratory syndrome virus by the polymerase chain reaction.

P Van Woensel1, J Van der Wouw, N Visser.   

Abstract

A polymerase chain reaction (PCR) is described for the detection of the porcine reproductive respiratory syndrome virus (PRRSV). Using the PCR we were able to detect about 30 infectious units of PRRSV resolved in tissue culture medium or sperm. For the detection of PRRSV in sperm the PCR is 10-times more sensitive than culturing in alveolar macrophages. For the detection of PRRSV, PCR provides a good alternative to cell culture methods.

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Year:  1994        PMID： 8071416     DOI： 10.1016/0166-0934(94)90024-8

Source DB:  PubMed          Journal:  J Virol Methods        ISSN： 0166-0934            Impact factor:   2.014


  6 in total

1.  Typing of porcine reproductive and respiratory syndrome viruses by a multiplex PCR assay.

Authors:  S A Gilbert; R Larochelle; R Magar; H J Cho; D Deregt
Journal:  J Clin Microbiol       Date:  1997-01       Impact factor: 5.948

Review 2.  Viruses in the mammalian male genital tract and their effects on the reproductive system.

Authors:  N Dejucq; B Jégou
Journal:  Microbiol Mol Biol Rev       Date:  2001-06       Impact factor: 11.056

3.  Detection of porcine reproductive and respiratory syndrome virus in boar semen by PCR.

Authors:  J Christopher-Hennings; E A Nelson; J K Nelson; R J Hines; S L Swenson; H T Hill; J J Zimmerman; J B Katz; M J Yaeger; C C Chase
Journal:  J Clin Microbiol       Date:  1995-07       Impact factor: 5.948

4.  Reverse transcription loop-mediated isothermal amplification for the detection of highly pathogenic porcine reproductive and respiratory syndrome virus.

Authors:  Hao-tai Chen; Jie Zhang; De-hui Sun; Li-na Ma; Xiang-tao Liu; Kai Quan; Yong-sheng Liu
Journal:  J Virol Methods       Date:  2008-09-02       Impact factor: 2.014

5.  Sensitive detection and typing of porcine reproductive and respiratory syndrome virus by RT-PCR amplification of whole viral genes.

Authors:  M B Oleksiewicz; A Bøtner; K G Madsen; T Storgaard
Journal:  Vet Microbiol       Date:  1998-11       Impact factor: 3.293

Review 6.  Porcine reproductive and respiratory syndrome (PRRS): a review, with emphasis on pathological, virological and diagnostic aspects.

Authors:  S H Done; D J Paton; M E White
Journal:  Br Vet J       Date:  1996-03
  6 in total

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