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Literature DB >> 8071232

Purification of the LysR family regulator, ClcR, and its interaction with the Pseudomonas putida clcABD chlorocatechol operon promoter.

Abstract

Previous studies have shown that the clcABD operon is under the transcriptional control of the LysR-type activator ClcR. In this study, the conditions leading to its aggregation were avoided and ClcR was purified and confirmed by amino-terminal sequencing. Gel filtration indicated that ClcR exists as a dimer in solution. The DNase I footprint of ClcR was determined. The binding properties of ClcR and the catechol operon regulator, CatR, were compared.

Entities: Chemical Species

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Year: 1994 PMID： 8071232 PMCID： PMC196742 DOI： 10.1128/jb.176.17.5530-5533.1994

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

15 in total

1. Predicting optimal and suboptimal secondary structure for RNA.

Authors: J A Jaeger; D H Turner; M Zuker
Journal: Methods Enzymol Date: 1990 Impact factor: 1.600

2. Functional analysis of the Pseudomonas putida regulatory protein CatR: transcriptional studies and determination of the CatR DNA-binding site by hydroxyl-radical footprinting.

Authors: R K Rothmel; D L Shinabarger; M R Parsek; T L Aldrich; A M Chakrabarty
Journal: J Bacteriol Date: 1991-08 Impact factor: 3.490

3. Precipitation techniques.

Authors: S Englard; S Seifter
Journal: Methods Enzymol Date: 1990 Impact factor: 1.600

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Authors: J A Jaeger; D H Turner; M Zuker
Journal: Proc Natl Acad Sci U S A Date: 1989-10 Impact factor: 11.205

5. Characterization of the Pseudomonas sp. strain P51 gene tcbR, a LysR-type transcriptional activator of the tcbCDEF chlorocatechol oxidative operon, and analysis of the regulatory region.

Authors: J R van der Meer; A C Frijters; J H Leveau; R I Eggen; A J Zehnder; W M de Vos
Journal: J Bacteriol Date: 1991-06 Impact factor: 3.490

6. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.

Authors: C Yanisch-Perron; J Vieira; J Messing
Journal: Gene Date: 1985 Impact factor: 3.688

7. Conserved motifs in a divergent nod box of Azorhizobium caulinodans ORS571 reveal a common structure in promoters regulated by LysR-type proteins.

Authors: K Goethals; M Van Montagu; M Holsters
Journal: Proc Natl Acad Sci U S A Date: 1992-03-01 Impact factor: 11.205

8. A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes.

Authors: S Tabor; C C Richardson
Journal: Proc Natl Acad Sci U S A Date: 1985-02 Impact factor: 11.205

9. Roles of CatR and cis,cis-muconate in activation of the catBC operon, which is involved in benzoate degradation in Pseudomonas putida.

Authors: M R Parsek; D L Shinabarger; R K Rothmel; A M Chakrabarty
Journal: J Bacteriol Date: 1992-12 Impact factor: 3.490

10. Characterization of Acinetobacter calcoaceticus catM, a repressor gene homologous in sequence to transcriptional activator genes.

Authors: E L Neidle; C Hartnett; L N Ornston
Journal: J Bacteriol Date: 1989-10 Impact factor: 3.490

13 in total

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Authors: Becky M Bundy; Lauren S Collier; Timothy R Hoover; Ellen L Neidle
Journal: Proc Natl Acad Sci U S A Date: 2002-05-28 Impact factor: 11.205

2. Inactivation of the nod box distal half-site allows tetrameric NodD to activate nodA transcription in an inducer-independent manner.

Authors: Jie Feng; Qiang Li; Hai-Liang Hu; Xiao-Chun Chen; Guo-Fan Hong
Journal: Nucleic Acids Res Date: 2003-06-15 Impact factor: 16.971

Review 3. Bacterial transcriptional regulators for degradation pathways of aromatic compounds.

Authors: David Tropel; Jan Roelof van der Meer
Journal: Microbiol Mol Biol Rev Date: 2004-09 Impact factor: 11.056

4. catM encodes a LysR-type transcriptional activator regulating catechol degradation in Acinetobacter calcoaceticus.

Authors: C E Romero-Arroyo; M A Schell; G L Gaines; E L Neidle
Journal: J Bacteriol Date: 1995-10 Impact factor: 3.490

5. Characterization of TsaR, an oxygen-sensitive LysR-type regulator for the degradation of p-toluenesulfonate in Comamonas testosteroni T-2.

Authors: Tewes Tralau; Jörg Mampel; Alasdair M Cook; Jürgen Ruff
Journal: Appl Environ Microbiol Date: 2003-04 Impact factor: 4.792

6. 2-chloromuconate and ClcR-mediated activation of the clcABD operon: in vitro transcriptional and DNase I footprint analyses.

Authors: S M McFall; M R Parsek; A M Chakrabarty
Journal: J Bacteriol Date: 1997-06 Impact factor: 3.490

7. Transcriptional activation of the chlorocatechol degradative genes of Ralstonia eutropha NH9.

Authors: N Ogawa; S M McFall; T J Klem; K Miyashita; A M Chakrabarty
Journal: J Bacteriol Date: 1999-11 Impact factor: 3.490

8. Interaction of two LysR-type regulatory proteins CatR and ClcR with heterologous promoters: functional and evolutionary implications.

Authors: M R Parsek; S M McFall; D L Shinabarger; A M Chakrabarty
Journal: Proc Natl Acad Sci U S A Date: 1994-12-20 Impact factor: 11.205

9. The clc element of Pseudomonas sp. strain B13, a genomic island with various catabolic properties.

Authors: Muriel Gaillard; Tatiana Vallaeys; Frank Jörg Vorhölter; Marco Minoia; Christoph Werlen; Vladimir Sentchilo; Alfred Pühler; Jan Roelof van der Meer
Journal: J Bacteriol Date: 2006-03 Impact factor: 3.490

10. CatM regulation of the benABCDE operon: functional divergence of two LysR-type paralogs in Acinetobacter baylyi ADP1.

Authors: Obidimma C Ezezika; Lauren S Collier-Hyams; Haley A Dale; Andrew C Burk; Ellen L Neidle
Journal: Appl Environ Microbiol Date: 2006-03 Impact factor: 4.792