Literature DB >> 8068751

Comparison of immunohistochemistry, acid-fast staining, and cultivation for detection of Mycobacterium paratuberculosis in goats.

O F Thoresen1, K Falk, O Evensen.   

Abstract

Forty-seven paired specimens of ileum and mesenterial lymph nodes from goats originating from 2 herds with paratuberculosis were investigated. Culture of the specimens for Mycobacterium paratuberculosis was compared with Ziehl-Neelsen staining and with immunohistochemical tests on paraffin-embedded tissue sections, using a M. paratuberculosis immune serum and an avidin-biotin-alkaline phosphatase method. Immunohistochemical techniques detected most positive samples and produced more clearly visible reactions than did acid-fast staining. Eighteen of 47 samples were positive by immunohistochemical techniques may represent a valuable adjunct to standard techniques for diagnosis of paratuberculosis in goats.

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Year:  1994        PMID： 8068751     DOI： 10.1177/104063879400600210

Source DB:  PubMed          Journal:  J Vet Diagn Invest        ISSN： 1040-6387            Impact factor:   1.279


  7 in total

1.  Diagnosis of paratuberculosis in goats by cell mediated immune response, conventional and molecular diagnostic techniques.

Authors:  Sulficar Shamsudeen Rawther; Mapranath Raghavan Saseendranath; Govinda Pilla Krishnan Nair; Palathingal Vareid Tresamol; Usha Narayana Pillai; John Abraham; Tuticorin Maragatham Alagesan Senthilkumar; Shrine Nagalakshmy; Krishna Kripa Narayanan Nimisha
Journal:  Trop Anim Health Prod       Date:  2011-09-29       Impact factor: 1.559

2.  Duplex PCR for differential identification of Mycobacterium bovis, M. avium, and M. avium subsp. paratuberculosis in formalin- fixed paraffin-embedded tissues from cattle.

Authors:  C Coetsier; P Vannuffel; N Blondeel; J F Denef; C Cocito; J L Gala
Journal:  J Clin Microbiol       Date:  2000-08       Impact factor: 5.948

3.  Detection of Mycobacterium avium subsp. paratuberculosis in infected tissues by new species-specific immunohistological procedures.

Authors:  C Coetsier; X Havaux; F Mattelard; S Sadatte; F Cormont; K Buergelt; B Limbourg; D Latinne; H Bazin; J F Denef; C Cocito
Journal:  Clin Diagn Lab Immunol       Date:  1998-07

4.  Development and characterization of monoclonal antibodies and aptamers against major antigens of Mycobacterium avium subsp. paratuberculosis.

Authors:  John P Bannantine; Thomas J Radosevich; Judith R Stabel; Srinand Sreevatsan; Vivek Kapur; Michael L Paustian
Journal:  Clin Vaccine Immunol       Date:  2007-03-07

5.  Application of antibodies to recombinant heat shock protein 70 in immunohistochemical diagnosis of mycobacterium avium subspecies paratuberculosis in tissues of naturally infected cattle.

Authors:  Julius Boniface Okuni; David Patrick Kateete; Moses Okee; Anna Nanteza; Moses Joloba; Lonzy Ojok
Journal:  Ir Vet J       Date:  2017-03-24       Impact factor: 2.146

6.  Comparison of immunohistochemistry and Ziehl-Neelsen staining for detecting the distribution of Mycobacterium avium subsp avium in naturally infected domestic Pekin ducks (Anas platyrhynchos domestica).

Authors:  Dekang Zhu; Hongxi Chen; Xumin Ou; Mafeng Liu; Mingshu Wang; Xinxin Zhao; Renyong Jia; Shun Chen; Kunfeng Sun; Qiao Yang; Ying Wu; Xiaoyue Chen; Anchun Cheng
Journal:  Vet Med Sci       Date:  2019-11-26

Review 7.  Moving towards improved surveillance and earlier diagnosis of aquatic pathogens: From traditional methods to emerging technologies.

Authors:  Scott MacAulay; Amy R Ellison; Peter Kille; Joanne Cable
Journal:  Rev Aquac       Date:  2022-03-19
  7 in total

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