| Literature DB >> 8064861 |
R K Strong1, D E Vaughn, P J Bjorkman, P M Snow.
Abstract
A truncated form of Drosophila fasciclin III has been engineered by site-directed mutagenesis. Secreted fasciclin III is expressed at 35 to 40 mg/l in insect cells with baculovirus carrying the recombinant gene. Single crystals of purified soluble fasciclin III have been grown by vapor diffusion versus polyethylene glycol 8000/sodium citrate at low pH. The space group is P6(1)22 or its enantiomorph P6(5)22, with unit cell dimensions a = b = 140 A, c = 260 A. Cryo-preserved crystals diffract to reciprocal lattice spacings beyond 3.0 A.Entities:
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Year: 1994 PMID： 8064861 DOI： 10.1006/jmbi.1994.1523
Source DB: PubMed Journal: J Mol Biol ISSN： 0022-2836 Impact factor: 5.469