Literature DB >> 8064860

Purification, characterization and crystallization of human platelet profilin expressed in Escherichia coli.

A A Fedorov1, T D Pollard, S C Almo.   

Abstract

Human platelet profilin was expressed in Escherichia coli using a T7 based expression vector. The recombinant material is similar to authentic human platelet profilin based on the measured Kd for rabbit skeletal muscle actin. Crystals of the recombinant material were obtained from both PEG 8000 and (NH4)2SO4. These crystals are isomorphous and belong to the monoclinic space group C2, a = 75.0, b = 32.0, c = 62.5, beta = 123 degrees. These crystals contain one molecule in the asymmetric unit and diffract to at least 2.0 A.

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Year:  1994        PMID： 8064860     DOI： 10.1006/jmbi.1994.1522

Source DB:  PubMed          Journal:  J Mol Biol        ISSN： 0022-2836            Impact factor:   5.469


  23 in total

1.  Actin polymerization is essential for pollen tube growth.

Authors:  L Vidali; S T McKenna; P K Hepler
Journal:  Mol Biol Cell       Date:  2001-08       Impact factor: 4.138

2.  A proline-rich protein binds to the localization element of Xenopus Vg1 mRNA and to ligands involved in actin polymerization.

Authors:  W M Zhao; C Jiang; T T Kroll; P W Huber
Journal:  EMBO J       Date:  2001-05-01       Impact factor: 11.598

3.  Villin severing activity enhances actin-based motility in vivo.

Authors:  Céline Revenu; Matthieu Courtois; Alphée Michelot; Cécile Sykes; Daniel Louvard; Sylvie Robine
Journal:  Mol Biol Cell       Date:  2006-12-20       Impact factor: 4.138

4.  Characterization of maize (Zea mays) pollen profilin function in vitro and in live cells.

Authors:  B C Gibbon; H Ren; C J Staiger
Journal:  Biochem J       Date:  1997-11-01       Impact factor: 3.857

5.  The formin homology 1 domain modulates the actin nucleation and bundling activity of Arabidopsis FORMIN1.

Authors:  Alphée Michelot; Christophe Guérin; Shanjin Huang; Mathieu Ingouff; Stéphane Richard; Natalia Rodiuc; Christopher J Staiger; Laurent Blanchoin
Journal:  Plant Cell       Date:  2005-07-01       Impact factor: 11.277

6.  Profilin is required for optimal actin-dependent transcription of respiratory syncytial virus genome RNA.

Authors:  E Burke; N M Mahoney; S C Almo; S Barik
Journal:  J Virol       Date:  2000-01       Impact factor: 5.103

7.  Scar, a WASp-related protein, activates nucleation of actin filaments by the Arp2/3 complex.

Authors:  L M Machesky; R D Mullins; H N Higgs; D A Kaiser; L Blanchoin; R C May; M E Hall; T D Pollard
Journal:  Proc Natl Acad Sci U S A       Date:  1999-03-30       Impact factor: 11.205

Review 8.  Cofilin and profilin: partners in cancer aggressiveness.

Authors:  Joelle V F Coumans; Rhonda J Davey; Pierre D J Moens
Journal:  Biophys Rev       Date:  2018-07-19

9.  Structural basis for mutation-induced destabilization of profilin 1 in ALS.

Authors:  Sivakumar Boopathy; Tania V Silvas; Maeve Tischbein; Silvia Jansen; Shivender M Shandilya; Jill A Zitzewitz; John E Landers; Bruce L Goode; Celia A Schiffer; Daryl A Bosco
Journal:  Proc Natl Acad Sci U S A       Date:  2015-06-08       Impact factor: 11.205

10.  Actin Purified from Maize Pollen Functions in Living Plant Cells.

Authors:  H. Ren; B. C. Gibbon; S. L. Ashworth; D. M. Sherman; M. Yuan; C. J. Staiger
Journal:  Plant Cell       Date:  1997-08       Impact factor: 11.277
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