Literature DB >> 8063785

A novel protein interacts with the major transforming growth factor-beta responsive element in the plasminogen activator inhibitor type-1 gene.

M A Sandler1, J N Zhang, D R Westerhausen, J J Billadello.   

Abstract

Multiple transforming growth factor-beta (TGF-beta) responsive elements have been identified within the 5'-flanking region of the plasminogen activator inhibitor type-1 (PAI-1) gene. This study was designed to characterize the major TGF-beta responsive element (-804 to -546). DNA footprint assays showed that the region of protein contact (-726 to -703) did not include consensus sequences for any known transacting factors. The results of UV cross-linking and Southwestern blot experiments showed that a protein of M(r) 100,000 specifically binds to the TGF-beta responsive element and that this protein undergoes post-transcriptional activation within 5 min after stimulation of Hep G2 cells by TGF-beta resulting in a marked increase in affinity for the target DNA sequence. These results show that stimulation of Hep G2 cells with TGF-beta increases the affinity of a novel 100-kDa protein for the major TGF-beta responsive element within the PAI-1 gene.

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Year:  1994        PMID： 8063785

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  8 in total

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Journal:  J Clin Pathol       Date:  1999-12       Impact factor: 3.411

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Authors:  Catherine E Winbanks; Bo Wang; Claudia Beyer; Phillip Koh; Lloyd White; Phillip Kantharidis; Paul Gregorevic
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4.  Tyrosine dephosphorylation of nuclear proteins mimics transforming growth factor beta 1 stimulation of alpha 2(I) collagen gene expression.

Authors:  P Greenwel; W Hu; R A Kohanski; F Ramirez
Journal:  Mol Cell Biol       Date:  1995-12       Impact factor: 4.272

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Authors:  Eduardo Méndez; John R Houck; David R Doody; Wenhong Fan; Pawadee Lohavanichbutr; Tessa C Rue; Bevan Yueh; Neal D Futran; Melissa P Upton; D Gregory Farwell; Patrick J Heagerty; Lue Ping Zhao; Stephen M Schwartz; Chu Chen
Journal:  Clin Cancer Res       Date:  2009-02-15       Impact factor: 12.531

6.  Identification of a potential regulator of early transcriptional responses to mesoderm inducers in the frog embryo.

Authors:  H C Huang; L C Murtaugh; P D Vize; M Whitman
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7.  SFRP1 reduction results in an increased sensitivity to TGF-β signaling.

Authors:  Kelly J Gauger; Kerry L Chenausky; Molly E Murray; Sallie S Schneider
Journal:  BMC Cancer       Date:  2011-02-08       Impact factor: 4.638

8.  Diversity of function is inherent in matricellular proteins: an appraisal of thrombospondin 1.

Authors:  P Bornstein
Journal:  J Cell Biol       Date:  1995-08       Impact factor: 10.539
  8 in total

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